IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/11: Difference between revisions

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(Autocreate 2013/07/11 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM)
 
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week Six</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==Lab==
* Insert your content here.
 
Cultures were retrieved from incubation and minipreps were performed following the protocol ''fig 2''. This was followed by a restriction enzyme digest reactions using Xba1 and Nde1 enzymes each relevant to its DNA .After incubation samples were run on a gel ''fig 3''to confirm the results.
 
[[Image:2013-07-11 cut sites confirmation.jpg|thumb|Fig 3: Analysis of restriction digest. Antgp plasmid cut with Xba1 and J04450 plasmid cut with Nde1 in lanes 1 and 2 respectively. Lanes 3-8 contain uncut samples 2a,2b,2c,3a,3b,3c respectively . Lanes 10-15 contain digested samples 2a,2b,2c, with Xba1 3a,3b,3c  with Nde1 respectively.]]
 
[[Image:FMHw4NaVv1qk6t2gg07NMBDmEMwdn6mFeHg2ZQIP6NU.jpg|thumb|Fig 2:Cultures grown overnight after inoculation of 3 colonies from the J04450-Nde1 plate (3a,b,c), showing a pink colour with slightly different intensities. ]]
 
 
The four cultures for the protein expression were recovered and centrifuged to provide a pellet, yielding eight pellets, two from each culture.
 
==Outreach==
As the UK meet up was the following day, we practiced and finalised our presentation.  




Revision as of 11:28, 22 August 2013

Week Six <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Lab

Cultures were retrieved from incubation and minipreps were performed following the protocol fig 2. This was followed by a restriction enzyme digest reactions using Xba1 and Nde1 enzymes each relevant to its DNA .After incubation samples were run on a gel fig 3to confirm the results.

Fig 3: Analysis of restriction digest. Antgp plasmid cut with Xba1 and J04450 plasmid cut with Nde1 in lanes 1 and 2 respectively. Lanes 3-8 contain uncut samples 2a,2b,2c,3a,3b,3c respectively . Lanes 10-15 contain digested samples 2a,2b,2c, with Xba1 3a,3b,3c with Nde1 respectively.
Fig 2:Cultures grown overnight after inoculation of 3 colonies from the J04450-Nde1 plate (3a,b,c), showing a pink colour with slightly different intensities.


The four cultures for the protein expression were recovered and centrifuged to provide a pellet, yielding eight pellets, two from each culture.

Outreach

As the UK meet up was the following day, we practiced and finalised our presentation.



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