IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/05: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Lucy Clark (talk | contribs) (Autocreate 2013/07/05 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM) |
Lucy Clark (talk | contribs) No edit summary |
||
| (4 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
{|{{table}} width="800" | {|{{table}} width="800" | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week Five</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
The plasmid pET-sigmaAntA, which expresses the His-tagged version of the AntA sigma factor, was transformed into both standard E.coli cells and E.coli used for protein (Rosetta BL21). Two plates containing the antibiotic Kanamycin and LB agar were spread with the sample containing standard E.coli cells. A further two plates containing antibiotics kanamycin and Chlorophenical and LB agar were spread with the BL21 E.coli sample. The four plates were left to incubate at room temperature for a couple of days. | |||
==Outreach== | |||
Soil tokens were ordered in business card. They were to be given to anyone donating a soil sample so they could track its progress and view the results of the screening. | |||
Revision as of 01:56, 25 July 2013
 Week Five
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
|
The plasmid pET-sigmaAntA, which expresses the His-tagged version of the AntA sigma factor, was transformed into both standard E.coli cells and E.coli used for protein (Rosetta BL21). Two plates containing the antibiotic Kanamycin and LB agar were spread with the sample containing standard E.coli cells. A further two plates containing antibiotics kanamycin and Chlorophenical and LB agar were spread with the BL21 E.coli sample. The four plates were left to incubate at room temperature for a couple of days. OutreachSoil tokens were ordered in business card. They were to be given to anyone donating a soil sample so they could track its progress and view the results of the screening.
| |
