IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/05/06: Difference between revisions

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(Autocreate 2010/05/06 Entry for IGEM:UNAM_Genomics_Mexico/2009/Notebook/Wifi_coli)
 
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==Entry title==
=6th May 2010=
* Insert your content here.
=Advances=
==Modeling==
*Proportion/Bioparts (#Copies, promoter strength):  We need to take note about the efficiencies of
 
plasmids by systems. Problems with LRE, Lux Y  and lumacin. LuxY and Lumacin, will be synthesized
 
with a strong promoter.
 
*General formula (luminescence). Explain the general formula. Formula considers substrate-protein
 
concentration. The advantage of this formula is the literature related, in the article.  Formula
 
includes cytosolic  Luciferin. Formula lack of double luciferase activity. 
 
*Physical Design.  Related 4 April, link image.
 
*Team purposes make both physical designs, aisled (unidirectional) and open (multidirectional).
 
*Limits. Quantity of L-cystein, for this we purpose a rich medium of L-Cystein.
 
*Culture medium. Transparent, there are many options but in can be made.
 
===We need:===
 
Make a list for variables in model.
 
Re analyze the strength of promoter Vs copy number of plasmid.
 
Establish good proportions for concentrations in different bio-parts.
 
==WetLab==
*WetLab partners are going on in constructions.
 
==News==
 
*We will synthesize sequences in GenArt.
 
==Plans==
*Exposition with instructors.
 
*Ask for illuminometer.
 
 
==Other stuff==
Take note about importance of good Logbook.
 






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6th May 2010

Advances

Modeling

  • Proportion/Bioparts (#Copies, promoter strength): We need to take note about the efficiencies of

plasmids by systems. Problems with LRE, Lux Y and lumacin. LuxY and Lumacin, will be synthesized

with a strong promoter.

  • General formula (luminescence). Explain the general formula. Formula considers substrate-protein

concentration. The advantage of this formula is the literature related, in the article. Formula

includes cytosolic Luciferin. Formula lack of double luciferase activity.

  • Physical Design. Related 4 April, link image.
  • Team purposes make both physical designs, aisled (unidirectional) and open (multidirectional).
  • Limits. Quantity of L-cystein, for this we purpose a rich medium of L-Cystein.
  • Culture medium. Transparent, there are many options but in can be made.

We need:

Make a list for variables in model.

Re analyze the strength of promoter Vs copy number of plasmid.

Establish good proportions for concentrations in different bio-parts.

WetLab

  • WetLab partners are going on in constructions.

News

  • We will synthesize sequences in GenArt.

Plans

  • Exposition with instructors.
  • Ask for illuminometer.


Other stuff

Take note about importance of good Logbook.




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