IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/04/29: Difference between revisions

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Search for initial concentrations of Luciferin inoculated, and measure the decay of Luciferin trough the time.
Search for initial concentrations of Luciferin inoculated, and measure the decay of Luciferin trough the time.
Check Bionumbers web page.
Check Bionumbers web page.
Search for critical variables and stecheometric data.
Search for critical variables and stoichiometry data.




Revision as of 21:52, 5 May 2010

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29th April 2010

General

The structure of this logbook is divided in two sections, WetLab and modeling.

WetLab

Goals in April.

  • Test blue receptor-promoter (ycg F-E).
  • Mutate Luciferase, taste and measure it.
  • Construction of LovTAP
  • Test plasmids.
  • Synthesis of oligos.

Is necessary: Make right sequences to synthesize them. ATCC= Vibrio Fischery $254.

Modeling

Goals in April:

  • Know how our system can be analyzed and divided to make models for biologic and physical properties.
  • Understand LovTAP system because it will be the first biological device finished.

Is necessary: Search for initial concentrations of Luciferin inoculated, and measure the decay of Luciferin trough the time. Check Bionumbers web page. Search for critical variables and stoichiometry data.





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