IGEM:UC Berkeley/2006: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| (27 intermediate revisions by 6 users not shown) | |||
| Line 4: | Line 4: | ||
<div id="about"> | <div id="about"> | ||
{| | {| | ||
| rowspan=2 | [[Image:Berkeley.jpg|200px]] | | rowspan=2 | [[Image:Berkeley.jpg|left|200px]]'''Addressable Conjugation in Bacterial Networks''' | ||
Networks of interacting cells provide the basis for neural learning. We have developed the process of addressable conjugation for communication within a network of E. coli bacteria. Here, bacteria send messages to one another via conjugation of plasmid DNAs, but the message is only meaningful to cells with a matching address sequence. In this way, the Watson Crick base-pairing of addressing sequences replaces the spatial connectivity present in neural systems. To construct this system, we have adapted natural conjugation systems as the communication device. Information contained in the transferred plasmids is only accessable by "unlocking" the message using RNA based 'keys'. The resulting addressable conjugation process is being adapted to construct a network of NAND logic gates in bacterial cultures. Ultimately, this will allow us to develop networks of bacteria capable of trained learning. <br> | |||
'''The presentation of our results for the 2006 Jamboree is available at the [http://parts2.mit.edu/wiki/index.php/University_of_California_Berkeley_2006 iGEM Website]'''<br> | |||
We | [[Image:Microsoft_Logo.jpg|right|200 px]] | ||
[ | ''The Berkeley iGEM team very gratefully acknowledges the generous support of Microsoft, which helped make our team members' participation possible.''<br> | ||
|- | |- | ||
| Line 60: | Line 60: | ||
[[IGEM:UC Berkeley/2006/Procedure Overview (Plasmid DNA to Sequencing)|Procedure Overview (Plasmid DNA to Sequencing)]]<br> | [[IGEM:UC Berkeley/2006/Procedure Overview (Plasmid DNA to Sequencing)|Procedure Overview (Plasmid DNA to Sequencing)]]<br> | ||
[[IGEM:UC Berkeley/2006/Competent cell production|Competent cell production]]<br> | |||
[[IGEM:UC Berkeley/2006/Plasmid Transformation|Plasmid Transformation]]<br> | [[IGEM:UC Berkeley/2006/Plasmid Transformation|Plasmid Transformation]]<br> | ||
[[IGEM:UC Berkeley/2006/Digestion1|Digestion]]<br> | [[IGEM:UC Berkeley/2006/Digestion1|Digestion]]<br> | ||
[[IGEM:UC Berkeley/2006/Ligation|Ligation]]<br> | [[IGEM:UC Berkeley/2006/Ligation|Ligation]]<br> | ||
[[IGEM:UC Berkeley/2006/Sequencing|Sequencing]]<br> | [[IGEM:UC Berkeley/2006/Sequencing|Sequencing]]<br> | ||
[[IGEM:UC Berkeley/2006/ | [[IGEM:UC Berkeley/2006/OverlapExtensionRxn|Overlap extension of oligos]]<br> | ||
[[IGEM:UC Berkeley/2006/PCRPrep|PCR prep]]<br> | [[IGEM:UC Berkeley/2006/PCRPrep|PCR prep]]<br> | ||
[[IGEM:UC Berkeley/2006/Conjugation|Conjugation]]<br> | [[IGEM:UC Berkeley/2006/Conjugation|Conjugation]]<br> | ||
| Line 71: | Line 72: | ||
[[IGEM:UC Berkeley/2006/PCR machine program (extend kit)|PCR machine program (Expand kit)]]<br> | [[IGEM:UC Berkeley/2006/PCR machine program (extend kit)|PCR machine program (Expand kit)]]<br> | ||
[[IGEM:UC Berkeley/2006/Knockouts|Knockouts]]<br> | [[IGEM:UC Berkeley/2006/Knockouts|Knockouts]]<br> | ||
[[IGEM:UC Berkeley/2006/Knockouts by Electroporation of pOX38 x pKD46|Knockouts by Electroporation of pOX38 x pKD46]]<br> | |||
| Line 76: | Line 78: | ||
[[Berk2006-ConjugationTeam | Conjugation 6/7/06-7/21/06]]<br> | [[Berk2006-ConjugationTeam | Conjugation 6/7/06-7/21/06]]<br> | ||
[[Berk2006-ConjugationTeam2 | Conjugation 7/24/06 - present]]<br> | [[Berk2006-ConjugationTeam2 | Conjugation 7/24/06 - 09/07/06]]<br> | ||
[[Berk2006-ConjugationTeam3 | Conjugation 09/07/06- present]]<br> | |||
[[Berk2006-LocksAndKeysTeam | Locks and Keys]]<br> | [[Berk2006-LocksAndKeysTeam | Locks and Keys]]<br> | ||
[[Berk2006-LogicGatesTeam | Logic Gates]]<br> | [[Berk2006-LogicGatesTeam | Logic Gates]]<br> | ||
[http://www.openwetware.org/wiki/IGEM:UC_Berkeley/2006/bryans_notebook Bryan's Lock and Keys]<br> | [http://www.openwetware.org/wiki/IGEM:UC_Berkeley/2006/bryans_notebook Bryan's Lock and Keys]<br> | ||
[[Berk2006-Samantha's Notebook | Samantha's Notebook]]<br> | |||
<br> | <br> | ||
| Line 106: | Line 110: | ||
*[http://parts2.mit.edu/r/parts/partsdb/pgroup.cgi?pgroup=iGEM&group=iGEM_Berkeley 2005 Biobricks] | *[http://parts2.mit.edu/r/parts/partsdb/pgroup.cgi?pgroup=iGEM&group=iGEM_Berkeley 2005 Biobricks] | ||
*[[Image:iGEM2006Distribution.xls|iGEM2006 Distribution compiled list]] | *[[Image:iGEM2006Distribution.xls|iGEM2006 Distribution compiled list]] | ||
*[http://parts2.mit.edu/wiki/index.php/University_of_California_Berkeley_2006 | iGEM site] | |||
'''Subgroup Strategies, Overview''' | |||
*[[Powerpoint presentations, drafts]] | |||
|} | |} | ||
Latest revision as of 13:29, 4 June 2010
 Networks of interacting cells provide the basis for neural learning. We have developed the process of addressable conjugation for communication within a network of E. coli bacteria. Here, bacteria send messages to one another via conjugation of plasmid DNAs, but the message is only meaningful to cells with a matching address sequence. In this way, the Watson Crick base-pairing of addressing sequences replaces the spatial connectivity present in neural systems. To construct this system, we have adapted natural conjugation systems as the communication device. Information contained in the transferred plasmids is only accessable by "unlocking" the message using RNA based 'keys'. The resulting addressable conjugation process is being adapted to construct a network of NAND logic gates in bacterial cultures. Ultimately, this will allow us to develop networks of bacteria capable of trained learning.  The Berkeley iGEM team very gratefully acknowledges the generous support of Microsoft, which helped make our team members' participation possible. |
