IGEM:UC-Merced/2009/Notebook/E. hydro Express - Revision history

http://openwetware.org/index.php?title=IGEM:UC-Merced/2009/Notebook/E._hydro_Express&feed=atom&action=history IGEM:UC-Merced/2009/Notebook/E. hydro Express - Revision history 2013-05-19T08:51:30Z Revision history for this page on the wiki MediaWiki 1.13.2 http://openwetware.org/index.php?title=IGEM:UC-Merced/2009/Notebook/E._hydro_Express&diff=698324&oldid=prev Sunny Seth: /* Notes */ 2013-05-16T21:48:19Z

<p><span class="autocomment">Notes</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 21:48, 16 May 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 365:</td> <td colspan="2" class="diff-lineno">Line 365:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>05/08/2013</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>05/08/2013</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>1. Removed JW 1228 bacteria from incubator at 12:25pm</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>1. Removed JW 1228 bacteria from incubator at 12:25pm <ins class="diffchange diffchange-inline">and put into the 4C fridge</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">05/15/2013</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">1. Cells were removed from the 4C fridge.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">2. Steps were followed from the Promega MagaZorb kit up, with the only deviation being that 1xPBS buffer was used instead of 10xPBS buffer.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">3. Due to time constraints the last step that was performed was putting in 500mL of binding buffer.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">4. If the products from these steps are salvagable, they are in a tube labelled "Xtract CH" in the 4C fridge.</ins></div></td></tr>   </table>

Sunny Seth http://openwetware.org/index.php?title=IGEM:UC-Merced/2009/Notebook/E._hydro_Express&diff=697058&oldid=prev Sunny Seth: /* Notes */ 2013-05-08T19:34:17Z

<p><span class="autocomment">Notes</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 19:34, 8 May 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 362:</td> <td colspan="2" class="diff-lineno">Line 362:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. Added 50uL of JW 1228-1 from stock "Baij 11/4" from the 4 C fridge to 5mL of LB "8/27" and put in the incubator over night. Put in at 12:05pm</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. Added 50uL of JW 1228-1 from stock "Baij 11/4" from the 4 C fridge to 5mL of LB "8/27" and put in the incubator over night. Put in at 12:05pm</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">05/08/2013</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">1. Removed JW 1228 bacteria from incubator at 12:25pm</ins></div></td></tr>  </table>

Sunny Seth http://openwetware.org/index.php?title=IGEM:UC-Merced/2009/Notebook/E._hydro_Express&diff=696851&oldid=prev Sunny Seth: /* Notes */ 2013-05-07T19:09:11Z

<p><span class="autocomment">Notes</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 19:09, 7 May 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 358:</td> <td colspan="2" class="diff-lineno">Line 358:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. Plates were observed and there was not any change. This is not indicative of anything other than the colonies present are resistant to kanamycin</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. Plates were observed and there was not any change. This is not indicative of anything other than the colonies present are resistant to kanamycin</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">05/07/2013</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">1. Added 50uL of JW 1228-1 from stock "Baij 11/4" from the 4 C fridge to 5mL of LB "8/27" and put in the incubator over night. Put in at 12:05pm</ins></div></td></tr>  </table>

Sunny Seth http://openwetware.org/index.php?title=IGEM:UC-Merced/2009/Notebook/E._hydro_Express&diff=684941&oldid=prev Sunny Seth: /* Notes */ 2013-03-22T18:22:18Z

<p><span class="autocomment">Notes</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 18:22, 22 March 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 354:</td> <td colspan="2" class="diff-lineno">Line 354:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. A colony of cells were chosen on the 100ml of kanamycin plate. Two designated areas containing a high number of colonies was marked and then 5uL of kanamycin was pipetted onto that area in hopes that the presence of these cells did not represent a false positive.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. A colony of cells were chosen on the 100ml of kanamycin plate. Two designated areas containing a high number of colonies was marked and then 5uL of kanamycin was pipetted onto that area in hopes that the presence of these cells did not represent a false positive.</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">3/22/13</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">1. Plates were observed and there was not any change. This is not indicative of anything other than the colonies present are resistant to kanamycin</ins></div></td></tr>  </table>

Sunny Seth http://openwetware.org/index.php?title=IGEM:UC-Merced/2009/Notebook/E._hydro_Express&diff=684737&oldid=prev Sunny Seth: /* Notes */ 2013-03-21T18:32:42Z

<p><span class="autocomment">Notes</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 18:32, 21 March 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 334:</td> <td colspan="2" class="diff-lineno">Line 334:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3/15/13</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3/15/13</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>1. Transformed FMJ Culture was plated at concentration of 10 ml and 100 ml (two plates)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>1. Transformed FMJ Culture was plated at concentration of 10 ml and 100 ml <ins class="diffchange diffchange-inline">of kanamycin </ins>(two plates) <ins class="diffchange diffchange-inline">along with a control plate that only contained cells and not any of the antibiotic.</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2. The transformed culture plates are to be scored on 3/16/13.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2. The transformed culture plates are to be scored on 3/16/13.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td colspan="2" class="diff-lineno">Line 346:</td> <td colspan="2" class="diff-lineno">Line 346:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3/18/13</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3/18/13</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. The plates labeled Transduced FMJ + KM were removed from the incubator at 3:30 pm and scored.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. The plates labeled Transduced FMJ + KM were removed from the incubator at 3:30 pm and scored.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2. There a appears to be a large amount of microbial growth toward the edges of the plate where there was a smaller concentration of KM. There was no sign of microbial growth in the middle of the plate where the largest amount of KM would be present. There may be a false positive present since the Transduced FMJ was mixed with KM and then plated on LB plates instead of selective plates.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2. There a appears to be a large amount of microbial growth toward the edges of the plate where there was a smaller concentration of KM. There was no sign of microbial growth in the middle of the plate where the largest amount of KM would be present. There may be a false positive present since the Transduced FMJ was mixed with KM and then plated on LB plates instead of selective plates.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3. The experiment should be redone using KM selected plates to fix the above possible error.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3. The experiment should be redone using KM selected plates to fix the above possible error.</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">3/21/13</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">1. A colony of cells were chosen on the 100ml of kanamycin plate. Two designated areas containing a high number of colonies was marked and then 5uL of kanamycin was pipetted onto that area in hopes that the presence of these cells did not represent a false positive.</ins></div></td></tr>  </table>

Sunny Seth http://openwetware.org/index.php?title=IGEM:UC-Merced/2009/Notebook/E._hydro_Express&diff=683866&oldid=prev Sunny Seth: /* Notes */ 2013-03-18T22:36:44Z

<p><span class="autocomment">Notes</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 22:36, 18 March 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 344:</td> <td colspan="2" class="diff-lineno">Line 344:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>4. The cultures were replated with 20 microliters of Kanamycin to be scored on 3/18</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>4. The cultures were replated with 20 microliters of Kanamycin to be scored on 3/18</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>   A. Kanamycin was added to ensure that the cultures retained antibiotic resistance.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>   A. Kanamycin was added to ensure that the cultures retained antibiotic resistance.</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">3/18/13</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">1. The plates labeled Transduced FMJ + KM were removed from the incubator at 3:30 pm and scored.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">2. There a appears to be a large amount of microbial growth toward the edges of the plate where there was a smaller concentration of KM. There was no sign of microbial growth in the middle of the plate where the largest amount of KM would be present. There may be a false positive present since the Transduced FMJ was mixed with KM and then plated on LB plates instead of selective plates.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">3. The experiment should be redone using KM selected plates to fix the above possible error.</ins></div></td></tr>  </table>

Sunny Seth http://openwetware.org/index.php?title=IGEM:UC-Merced/2009/Notebook/E._hydro_Express&diff=683535&oldid=prev Sunny Seth at 20:03, 17 March 2013 2013-03-17T20:03:23Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:03, 17 March 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 14:</td> <td colspan="2" class="diff-lineno">Line 14:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|colspan="2"|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|colspan="2"|</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Description/Abstract==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Description/Abstract==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">* Place short description of project or notes regarding this project</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Energy production in modern society relies primarily on fossil fuels. However, fossil</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">fuels are a limited resource and their use generates pollution. Our goal is to create</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">* Lorem ipsum dolor sit amet, consectetuer adipiscing elit</del>. <del class="diffchange diffchange-inline">Donec porta commodo tellus. Nam </del>a <del class="diffchange diffchange-inline">est eget libero mollis tincidunt</del>. <del class="diffchange diffchange-inline">Aliquam purus. Quisque nulla ligula</del>, <del class="diffchange diffchange-inline">facilisis </del>in<del class="diffchange diffchange-inline">, pulvinar sed, molestie a, quam</del>. <del class="diffchange diffchange-inline">Vestibulum at pede</del>. <del class="diffchange diffchange-inline">In </del>in <del class="diffchange diffchange-inline">sem eget odio eleifend placerat. Phasellus ultricies felis quis sapien. Etiam molestie volutpat quam</del>. <del class="diffchange diffchange-inline">Praesent pulvinar scelerisque mi. Nam mi urna, fringilla eu, mattis sed, venenatis id</del>, <del class="diffchange diffchange-inline">nunc</del>. <del class="diffchange diffchange-inline">Maecenas velit eros</del>, <del class="diffchange diffchange-inline">congue ut</del>, <del class="diffchange diffchange-inline">placerat in, ornare vel, sem</del>. <del class="diffchange diffchange-inline">Aenean porta enim sit amet felis gravida posuere</del>. <del class="diffchange diffchange-inline">Phasellus faucibus nibh et orci</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">a sustainable and clean energy source to fossil fuel</ins>. <ins class="diffchange diffchange-inline">Hydrogen gas is considered </ins>a</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">clean alternative energy source</ins>. <ins class="diffchange diffchange-inline">To achieve this goal</ins>, <ins class="diffchange diffchange-inline">we engineered the</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">fermentation pathway </ins>in <ins class="diffchange diffchange-inline">E</ins>. <ins class="diffchange diffchange-inline">coli FMJ39 for H2 gas production</ins>. <ins class="diffchange diffchange-inline">This strain is mutated</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">for the ldhA and pflB genes, involved </ins>in <ins class="diffchange diffchange-inline">mixed acid fermentation</ins>. <ins class="diffchange diffchange-inline">Also</ins>, <ins class="diffchange diffchange-inline">we will</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">delete gene adhE by P1 phage generalized transduction</ins>. <ins class="diffchange diffchange-inline">Additionally</ins>, <ins class="diffchange diffchange-inline">we cloned</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">the genes of dark fermentation Acetaldehyde dehydrogenase (MphF) from</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Methylophilales bacterium</ins>, <ins class="diffchange diffchange-inline">and Ferredoxin oxidoreductase (FO) from E</ins>. <ins class="diffchange diffchange-inline">coli W and</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">transformed E</ins>. <ins class="diffchange diffchange-inline">coli FMJ39, creating a new pathway for optimal production of</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">hydrogen gas. Producing such an alternative fuel will address the rising issues of a</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">dwindling fossil fuel supply, and mitigate its demand as a future primary fuel source</ins>.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Notes==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Notes==</div></td></tr>   </table>

Sunny Seth http://openwetware.org/index.php?title=IGEM:UC-Merced/2009/Notebook/E._hydro_Express&diff=683404&oldid=prev Sunny Seth: /* Notes */ 2013-03-16T20:53:08Z

<p><span class="autocomment">Notes</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:53, 16 March 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 322:</td> <td colspan="2" class="diff-lineno">Line 322:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>   A. 0.147g of CaCl2 (10mM) and 0.124g of MgSO4 (5mM) were added to 100mL of water and then autoclaved on setting 9.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>   A. 0.147g of CaCl2 (10mM) and 0.124g of MgSO4 (5mM) were added to 100mL of water and then autoclaved on setting 9.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2. Used flasks and bottles were autoclaved.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2. Used flasks and bottles were autoclaved.</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">3/15/13</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">1. Transformed FMJ Culture was plated at concentration of 10 ml and 100 ml (two plates)</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">2. The transformed culture plates are to be scored on 3/16/13.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">3/16/13</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">1. The plates were scored.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">2. The control plate at bacterial growth, which was expected. The control contained pure FMJ strain with no Kanamycin.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">3. All other plates (1 10 ml, and 2 100 ml) showed bacterial growth.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">4. The cultures were replated with 20 microliters of Kanamycin to be scored on 3/18</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">  A. Kanamycin was added to ensure that the cultures retained antibiotic resistance.</ins></div></td></tr>  </table>

Sunny Seth http://openwetware.org/index.php?title=IGEM:UC-Merced/2009/Notebook/E._hydro_Express&diff=683148&oldid=prev Sunny Seth: /* Notes */ 2013-03-15T01:26:20Z

<p><span class="autocomment">Notes</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 01:26, 15 March 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 308:</td> <td colspan="2" class="diff-lineno">Line 308:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. Agar plates were made.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. Agar plates were made.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>   A. 500mL of agar was made. 3.5g of agar and 10g of a mixture containing tryptone(peptone instead), yeast extract, and NaCl was added to 500mL of water.  </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>   A. 500mL of agar was made. 3.5g of agar and 10g of a mixture containing tryptone(peptone instead), yeast extract, and NaCl was added to 500mL of water.  </div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>   B. The solution was stirred and then autoclaved at 12:39pm and retrieved at about 1:45pm and let cool.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>   B. The solution was stirred and then autoclaved at 12:39pm and retrieved at about 1:45pm and let cool<ins class="diffchange diffchange-inline">. </ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">  C. Once cool the agar and plates were brought to the fume hood that was sprayed down with 70% alcohol solution.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">  D. The plates were filled about halfway with the agar solution, swirled for evenness, and then let to solidify.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">  E. Once all the plates were poured they were put back in their bag and labeled "CH agar 03/13/2013" and put in the walk in freezer</ins>.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3/13/13</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3/13/13</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>   1. "FMJ 39 Baij" was removed from the incubator and placed in fridge at 8:09 am. There were visible signs of a large amount of microbial growth including the broth looking very turbid.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>   1. "FMJ 39 Baij" was removed from the incubator and placed in fridge at 8:09 am. There were visible signs of a large amount of microbial growth including the broth looking very turbid.</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">3/14/2013</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">1. P1 salts were made.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">  A. 0.147g of CaCl2 (10mM) and 0.124g of MgSO4 (5mM) were added to 100mL of water and then autoclaved on setting 9.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">2. Used flasks and bottles were autoclaved.</ins></div></td></tr>  </table>

Sunny Seth http://openwetware.org/index.php?title=IGEM:UC-Merced/2009/Notebook/E._hydro_Express&diff=682829&oldid=prev Sunny Seth: /* Notes */ 2013-03-13T15:18:08Z

<p><span class="autocomment">Notes</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 15:18, 13 March 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 302:</td> <td colspan="2" class="diff-lineno">Line 302:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>   3. The culture tube named "FMJ 39 Baij" was set into the incubator to incubate till 12 noon on 03/12/13</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>   3. The culture tube named "FMJ 39 Baij" was set into the incubator to incubate till 12 noon on 03/12/13</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>   4. The tubes were accidently removed at 6:30pm 03/11/13 and put into the fridge. On 03/12/13 these tubes were put back in the incubator at 12:15pm and will be retrieved at 8:15am 03/13/13.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>   4. The tubes were accidently removed at 6:30pm 03/11/13 and put into the fridge. On 03/12/13 these tubes were put back in the incubator at 12:15pm and will be retrieved at 8:15am 03/13/13.</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">    a. Possible contamination when removed early due to taking the cultures out and attempting to count them with a hemocytometer. </ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3/12/13</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3/12/13</div></td></tr> <tr><td colspan="2" class="diff-lineno">Line 308:</td> <td colspan="2" class="diff-lineno">Line 309:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>   A. 500mL of agar was made. 3.5g of agar and 10g of a mixture containing tryptone(peptone instead), yeast extract, and NaCl was added to 500mL of water.  </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>   A. 500mL of agar was made. 3.5g of agar and 10g of a mixture containing tryptone(peptone instead), yeast extract, and NaCl was added to 500mL of water.  </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>   B. The solution was stirred and then autoclaved at 12:39pm and retrieved at about 1:45pm and let cool.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>   B. The solution was stirred and then autoclaved at 12:39pm and retrieved at about 1:45pm and let cool.</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">3/13/13</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">  1. "FMJ 39 Baij" was removed from the incubator and placed in fridge at 8:09 am. There were visible signs of a large amount of microbial growth including the broth looking very turbid.</ins></div></td></tr>  </table>

Sunny Seth