IGEM:UC-Merced/2009/Notebook/E. hydro Express
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Phage | Phage | ||
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a. LB | a. LB | ||
b. Kanomycin to add to strain JW1228-1 | b. Kanomycin to add to strain JW1228-1 | ||
Revision as of 21:30, 13 November 2012
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Description/Abstract
Notes
Lab Notebook Beginning Tuesday November 13, 2012 Phage 1. Prepared Stocks
a. LB
b. Kanomycin to add to strain JW1228-1
C1V1=C2V2
25000 ug (V1) = 50ug (5ml)
C1 = 25000 ug --> kanomycin concentration
C2 = 50 ug, V2 = 5 ml broth
25000 ug (V1) - 250 ug (ml)
V1 = 0.01 ml = 10 uL --> kanomycin to add to strain JW1228-1
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Recent changes 19 May 2013
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