IGEM:UC-Merced/2009/Notebook/E. hydro Express

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 29: Line 29:
10/13/2012
10/13/2012
-
   Phage
+
   Generation P1 Phage Stock
-
   1. Prepared Stocks
+
   1. Prepared P1 Stock from Yale University
     a. LB
     a. LB
-
     b. Kanomycin to add to strain JW1228-1
+
     b. Kanamycin stock 25mg/ml to add to strain JW1228-1
         C1V1=C2V2
         C1V1=C2V2
-
       25000 ug (V1) = 50ug (5ml)
+
       25000 ug/ml (V1) = 50ug/ml (5ml)
-
       C1 = 25000 ug  --> kanomycin concentration
+
       C1 = 25000 ug/ml --> kanamycin concentration
-
       C2 = 50 ug,   V2 = 5 ml broth
+
       C2 = 50 ug/ml    
-
       25000 ug (V1) - 250 ug (ml)
+
      V2 = 5 ml broth
-
       V1 = 0.01 ml = 10 uL  --> kanomycin to add to strain JW1228-1  
+
       25000 ug/ml (V1) = 250 ug
 +
       V1 = 0.01 ml = 10 uL  --> kanamycin to add to strain JW1228-1  
       Incubate kanomycin in LB broth until 11am 10/14/12
       Incubate kanomycin in LB broth until 11am 10/14/12
Line 45: Line 46:
10/14/12
10/14/12
-
  Phage
+
P1 Phage
-
1. Overnight culture did not grow.  
+
1. Overnight culture did not grow. Possible reasons are: (1) The JW1228-1 culture used was old and found in the 4°C refrigerator. It is possible that the culture was no longer living. To address this possibility we will redo the procedure using frozen stock.  
-
2. Prepared new stock with frozen bacteria (procedure carried out as on 10/13/12)
+
2. Prepared new overnight culture with frozen JW1228-1 glycerol stock (procedure carried out as on 10/13/12)
-
    a. LB
+
    a. LB
-
     b. Kanomycin to add to strain JW1228-1
+
     b. Kanamycin stock 25mg/ml to add to strain JW1228-1
         C1V1=C2V2
         C1V1=C2V2
-
       25000 ug (V1) = 50ug (5ml)
+
       25000 ug/ml (V1) = 50ug/ml (5ml)
-
       C1 = 25000 ug  --> kanomycin concentration
+
       C1 = 25000 ug/ml --> kanamycin concentration
-
       C2 = 50 ug,   V2 = 5 ml broth
+
       C2 = 50 ug/ml    
-
       25000 ug (V1) - 250 ug (ml)
+
      V2 = 5 ml broth
-
       V1 = 0.01 ml = 10 uL  --> kanomycin to add to strain JW1228-1  
+
       25000 ug/ml (V1) = 250 ug
-
       Incubate kanomycin in LB broth until 11am 10/14/12
+
       V1 = 0.01 ml = 10 uL  --> kanamycin to add to strain JW1228-1  
 +
       Incubate kanomycin in LB broth until 10 am 10/15/12
      
      

Revision as of 20:46, 14 November 2012

Search this project

Customize your entry pages

Description/Abstract

  • Place short description of project or notes regarding this project
  • Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Donec porta commodo tellus. Nam a est eget libero mollis tincidunt. Aliquam purus. Quisque nulla ligula, facilisis in, pulvinar sed, molestie a, quam. Vestibulum at pede. In in sem eget odio eleifend placerat. Phasellus ultricies felis quis sapien. Etiam molestie volutpat quam. Praesent pulvinar scelerisque mi. Nam mi urna, fringilla eu, mattis sed, venenatis id, nunc. Maecenas velit eros, congue ut, placerat in, ornare vel, sem. Aenean porta enim sit amet felis gravida posuere. Phasellus faucibus nibh et orci.

Notes

  • Place some notes here for visitors
  • Example: Next team meeting on Tuesday, June 18th! Be there!

Lab Notebook

Beginning Tuesday November 13, 2012

10/13/2012 

 Generation P1 Phage Stock

  1. Prepared P1 Stock from Yale University
   a. LB
   b. Kanamycin stock 25mg/ml to add to strain JW1228-1
       C1V1=C2V2
      25000 ug/ml (V1) = 50ug/ml (5ml)
      C1 = 25000 ug/ml  --> kanamycin concentration
      C2 = 50 ug/ml   
      V2 = 5 ml broth
      25000 ug/ml (V1) = 250 ug
      V1 = 0.01 ml = 10 uL   --> kanamycin to add to strain JW1228-1 
     Incubate kanomycin in LB broth until 11am 10/14/12


10/14/12 

P1 Phage

1. Overnight culture did not grow. Possible reasons are: (1) The JW1228-1 culture used was old and found in the 4°C refrigerator. It is possible that the culture was no longer living. To address this possibility we will redo the procedure using frozen stock. 2. Prepared new overnight culture with frozen JW1228-1 glycerol stock (procedure carried out as on 10/13/12) 

    a. LB
   b. Kanamycin stock 25mg/ml to add to strain JW1228-1
       C1V1=C2V2
      25000 ug/ml (V1) = 50ug/ml (5ml)
      C1 = 25000 ug/ml  --> kanamycin concentration
      C2 = 50 ug/ml   
      V2 = 5 ml broth
      25000 ug/ml (V1) = 250 ug
      V1 = 0.01 ml = 10 uL   --> kanamycin to add to strain JW1228-1 
     Incubate kanomycin in LB broth until 10 am 10/15/12




Recent changes

16 May 2013

      17:48 IGEM:UC-Merced/2009/Notebook/E. hydro Express (diff; hist) . .  (+432) . . Sunny Seth (Talk | contribs) (→Notes: )



Retrieved from "http://openwetware.org/wiki/IGEM:UC-Merced/2009/Notebook/E._hydro_Express"
Personal tools