IGEM:UC-Merced/2009/Notebook/E. hydro Express

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Line 43: Line 43:
 +
10/14/12
 +
  Phage
 +
1. Overnight culture did not grow.
 +
2. Prepared new stock with frozen bacteria (procedure carried out as on 10/13/12)
 +
    a. LB
 +
    b. Kanomycin to add to strain JW1228-1
 +
        C1V1=C2V2
 +
      25000 ug (V1) = 50ug (5ml)
 +
      C1 = 25000 ug  --> kanomycin concentration
 +
      C2 = 50 ug,  V2 = 5 ml broth
 +
      25000 ug (V1) - 250 ug (ml)
 +
      V1 = 0.01 ml = 10 uL  --> kanomycin to add to strain JW1228-1
 +
      Incubate kanomycin in LB broth until 11am 10/14/12
      
      

Revision as of 20:32, 14 November 2012

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Lab Notebook

Beginning Tuesday November 13, 2012

10/13/2012 

 Phage

  1. Prepared Stocks
   a. LB
   b. Kanomycin to add to strain JW1228-1
       C1V1=C2V2
      25000 ug (V1) = 50ug (5ml)
      C1 = 25000 ug  --> kanomycin concentration
      C2 = 50 ug,   V2 = 5 ml broth
      25000 ug (V1) - 250 ug (ml)
      V1 = 0.01 ml = 10 uL   --> kanomycin to add to strain JW1228-1 
     Incubate kanomycin in LB broth until 11am 10/14/12


10/14/12 

 Phage

1. Overnight culture did not grow. 2. Prepared new stock with frozen bacteria (procedure carried out as on 10/13/12) 

   a. LB
   b. Kanomycin to add to strain JW1228-1
       C1V1=C2V2
      25000 ug (V1) = 50ug (5ml)
      C1 = 25000 ug  --> kanomycin concentration
      C2 = 50 ug,   V2 = 5 ml broth
      25000 ug (V1) - 250 ug (ml)
      V1 = 0.01 ml = 10 uL   --> kanomycin to add to strain JW1228-1 
     Incubate kanomycin in LB broth until 11am 10/14/12




Recent changes

19 May 2013

+      21:53 IGEM:UC-Merced/2009/Notebook/E. hydro Express‎ (2 changes) . . (+1,643) . . (Page history) [Sunny Seth‎ (2×)]



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