Biofilm Track
Eric F.
- Recieved S. Aureus from the Davies lab on a streaked plate. Strain is currently unknown, will know soon (through Rafael)
- Used the Day Culture protocol from common protocols
- Picked two colonies into two 14mL test tubes each containing 5mL LB broth
- Made one control containing just 5mL broth
- Incubated all 3 tubes at 37 degrees C for 9 hours
- Control remain clear, others were very cloudy
- Now being stored on the bench in a rack
QS Track
Jason and Eric F.
Competent Cell Transformation
- Tested the competent cells (DH5α and Db3.1 - made by Philip and Kevin) made yesterday using plasmid from last year labeled ASEM24(B) with Ampicilin resistance
- Tested one from each centrifuge tube, plated labeled B1, B2, B3, α1 ,α2
- Used the transformation protocol from common protocols
- In step 1, the competent cells were allowed to thaw for 1 hour on ice, and were occasionally hand warmed
- During step 2, noticed the α2 aliquot had too much volume, so we put it back in the -80 degree C and got a different one
- Note: This aliquot was hand heated more than the others and was only thawed on ice for 10 minutes
- 5 plates of transformed cells were spread onto Ampicilin plates and are incubating overnight in the biosafety room incubator at 37 degrees C
- Need to be removed by 1pm tomorrow
- Note: Need to plate more Amp plates tomorrow
- Note: There was no control, if there is no transformation, we should test the DNA with last year's competent cells
PCR Previously Transformed Cells
- Obtained results from the PCR of the transformed cells
- Results were inconclusive and confusing, the ladders showed no movement, only the dye moved (the dye moves independently right?)
- Picture is in the QS Track labbook
- Had trouble getting a clear picture
Misc.
Eric F.
- Removed culture of BW27783 from the incubator (30 degree) at 10am
- Control remained clear, tube containing E. Coli was quite cloudy
- Need to send to Tec-Monterrey, tubes are on the bench in a rack
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iGEM Project name 1
