IGEM:Team Bordeaux/2009/Notebook/Bacterial eyespot/2012/07/05
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< IGEM:Team Bordeaux/2009/Notebook | Bacterial eyespot | 2012 | 07(Difference between revisions)
(Autocreate 2012/07/05 Entry for IGEM:Team_Bordeaux/2009/Notebook/Bacterial_eyespot) |
Current revision (09:32, 17 July 2012) (view source) (→Day 3) |
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| - | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> | + | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Bacterial eyespot </span> |
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| - | == | + | ==Day 3== |
| - | + | The two plates 5.A and 5.B gave a good amount of colonies | |
| + | but 100μL is too much to inoculate | ||
| + | We picked single colonies and put them in 10 ml LB with Ampicilin 100mg/mL | ||
| + | And put the tube to grow at 30°C overnight | ||
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| + | The tube cultures seem to have gone well but again 1.E is slightly clearer than the others | ||
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| + | We precipitated tree 2mL tubes for each Biobrick by centrifugation 3min at 13000rpm | ||
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| + | We then made a miniprep for one of the tubes and froze the two tubes left | ||
| + | |||
| + | We used a QIAprep® Spin Miniprep kit from QIAGEN® [http://openwetware.org/wiki/Template:SBB-Protocols_Micro3] | ||
Current revision
 Bacterial eyespot
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Day 3The two plates 5.A and 5.B gave a good amount of colonies but 100μL is too much to inoculate We picked single colonies and put them in 10 ml LB with Ampicilin 100mg/mL And put the tube to grow at 30°C overnight The tube cultures seem to have gone well but again 1.E is slightly clearer than the others We precipitated tree 2mL tubes for each Biobrick by centrifugation 3min at 13000rpm We then made a miniprep for one of the tubes and froze the two tubes left We used a QIAprep® Spin Miniprep kit from QIAGEN® [1]
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