IGEM:Stanford/2010/Notebook/28 June 2010: Difference between revisions

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General Meeting
General Meeting
 
*Wiki stuff
-Transform the first wave of cells
**Everyone should sign up on 2010.igem.org website
--Pick colonies
***Take advantage of the new calendar system
--Colony PCR
***Need to transfer current website over
--Sequence parts to validate
**Current wiki needs a new vision
---Use the Smolke sequencing network
***Francisco + Alex assembling wiki committee
-Get competent cells
*Transform the first wave of cells
-Finalize sRNA library
**Pick colonies
**Colony PCR
**Sequence parts to validate
***Use the Smolke sequencing network
*Characterize promoters
**Digest, ligate
**Need to acquire enzymes
**Need an PO account.
***Email Jeanne
*Get competent cells
*Getting DNA
**Assembly PCR
**PCR from host cells
**Synthesize de novo
*Finalize sRNA library




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Revision as of 09:27, 28 June 2010

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Home Project Protocols Parts Notebook Team Archives

General Meeting

  • Wiki stuff
    • Everyone should sign up on 2010.igem.org website
      • Take advantage of the new calendar system
      • Need to transfer current website over
    • Current wiki needs a new vision
      • Francisco + Alex assembling wiki committee
  • Transform the first wave of cells
    • Pick colonies
    • Colony PCR
    • Sequence parts to validate
      • Use the Smolke sequencing network
  • Characterize promoters
    • Digest, ligate
    • Need to acquire enzymes
    • Need an PO account.
      • Email Jeanne
  • Get competent cells
  • Getting DNA
    • Assembly PCR
    • PCR from host cells
    • Synthesize de novo
  • Finalize sRNA library


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