IGEM:Stanford/2010/Notebook/15 April 2010: Difference between revisions
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[DNA-L] = [TotalDNA][L] / (kD + L) | [DNA-L] = [TotalDNA][L] / (kD + L) | ||
===Other oscillators=== | |||
====Repressilator==== | |||
Did not end up working too well, but still ground-breaking for its time. | |||
A ---| B ---| C ---| A | |||
The [http://www.nature.com/nature/journal/v403/n6767/abs/403335a0.html original paper]. | |||
[[wikipedia:Repressilator]] on Wikipedia. | |||
===See also=== | ===See also=== | ||
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Journal Club 4/15/10
Presentation by ...
DNA-ligand binding
d[DNA-L]/dt = k_on*[DNA][L] - k_off*[DNA-L]
If we define: [TotalDNA] = [DNA] + [DNA-L]
Then: d[DNA-L]/dt = k_on*([TotalDNA] - [DNA-L]) - k_off*[DNA-L]
Solving, we get: [DNA-L] = [TotalDNA][L] / (k_off/k_on + L)
Usually, we define the dissociation constant to be: kD = k_off/k_on
So we end up with: [DNA-L] = [TotalDNA][L] / (kD + L)
Other oscillators
Repressilator
Did not end up working too well, but still ground-breaking for its time.
A ---| B ---| C ---| A
The original paper. wikipedia:Repressilator on Wikipedia.
