IGEM:Slovenia HS 2015/2009/Notebook/Test/2015/07/13: Difference between revisions
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== | ==Plasmid isolation from medium== | ||
* | * Isolation was conducted accrdingly to [http://openwetware.org/wiki/FCCT_Biochemistry_Lab: Plasmid DNA purification using centrifuges]<br> | ||
==Control restriction== | |||
Mixture: | |||
* We have taken 5μL from each microcentrifuge tube | |||
* 0,55 ml of buffer | |||
* 0,2 μL of restriction enzyme Pst1 | |||
With the restriction we have linearized our plasmid. | |||
==Agarose gel electrophoresis== | |||
*We have conducted electrophoresis accordingly to [http://openwetware.org/wiki/FCCT_Biochemistry_Lab:Agarose_gel_electrophoresis Agarose gel electrophoresis]<br> | |||
*All restrictions were lain on the electrophoresis cell | |||
Revision as of 03:34, 26 July 2015
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Plasmid isolation from medium
Control restrictionMixture:
With the restriction we have linearized our plasmid. Agarose gel electrophoresis
|