IGEM:Slovenia HS 2015/2009/Notebook/Test/2015/04/22: Difference between revisions
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Anze Vozelj (talk | contribs) |
Anze Vozelj (talk | contribs) |
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* 100x diluted DNA | * 100x diluted DNA | ||
=Mixture for PCR reaction (master mix)= | |||
::{| border="1" cellpadding="2" cellspacing="0" | ::{| border="1" cellpadding="2" cellspacing="0" | ||
!component||||quantity (µL)|| | !component||||quantity (µL)|| | ||
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|Vtot.||/||190|| | |Vtot.||/||190|| | ||
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[[Image:20140423 (3).png|thumb| | =Electrophoresis= | ||
The next step was Agarose gel electrophoresis. We can see that there are some of the products, but they do not correspond of all the masses of the products. CfAB and BdhA are both 1000 bp long. 16S is 600 bp long. The reaction have to be repeated. | |||
===== [[Image:20140423 (3).png|thumb|left|350px|Agarose electrophoresis: 1-broth, 2-100x diluted broth, 3-DNA, 4-100x diluted DNA]]===== | |||
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Gene isolation
Isolation sources:
Mixture for PCR reaction (master mix)
ElectrophoresisThe next step was Agarose gel electrophoresis. We can see that there are some of the products, but they do not correspond of all the masses of the products. CfAB and BdhA are both 1000 bp long. 16S is 600 bp long. The reaction have to be repeated. |