IGEM:PennState/Labbook/Nimrah Ahmed: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Nimrah Ahmed (talk | contribs) mNo edit summary |
Nimrah Ahmed (talk | contribs) m (→Circuit) |
||
| Line 14: | Line 14: | ||
=====Circuit===== | =====Circuit===== | ||
O -> A | O -> A | ||
A+B -> C, GFP | A+B -> C, GFP | ||
A+C -> B, YFP | A+C -> B, YFP | ||
*Degradation tags on A, B, C, GFP, YFP, strongest on A, weakest on B and C. | *Degradation tags on A, B, C, GFP, YFP, strongest on A, weakest on B and C. | ||
*Intended result: shake once: GFP, shake again: YFP, shake again, GFP, etc. | *Intended result: shake once: GFP, shake again: YFP, shake again, GFP, etc. | ||
Revision as of 09:24, 15 May 2008
Meeting 5/15/2008
Circuit project ideas:
- Genetic Circuit basics: http://www.princeton.edu/~rweiss/bio-programming/weiss-natural-computing-2003.pdf
Sequential cell color change
- ex. Green->Red->Yellow->repeat.
- System: organic layer & bacterial aquatic layer in liquid form. Shake to combine organic layer w/ bacterial aquatic layer -> initiate color change.
- Structure: Messenger protein downstream of color protein, downstream of organic molecule-activated promoter
Components
- Organic compound: hydrophobic, heavy (won't evaporate), activates promoter
- Promoter: lac, responds to organic
- Color proteins
Circuit
O -> A
A+B -> C, GFP
A+C -> B, YFP
- Degradation tags on A, B, C, GFP, YFP, strongest on A, weakest on B and C.
- Intended result: shake once: GFP, shake again: YFP, shake again, GFP, etc.
Options:
- Bioluminescense : proteins like "M-Cherry" instead of flourescent proteins...
- Rather than shake, stir by thermal convection or a magnetic stirrer
(Peking U did something like this--sequential color change--but UV activated. MIT team idea: bacterial lava lamp?)
