June 20th, 2007: 9:45-5:00

Troubleshooting experiments
- bizarre negative control on enzyme assays suggest that some restriction occured
- all transformations failed
  - Single unifying trait of expirements: amp
Laboratory management
- Set up anhydrous containers for 100% ethanol and kanamycin
- Need to buy agarose for Dr.Richard's lab
- Began processing of switching to SYBR green instead of ethidium bromide
  - SYBR is less mutagenic, may have better results, and would use dual purpose (GFP) filter
  - Real time visualization
  - No UV, less damage to DNA and safre to use
  - Pre stain, less toxic, .3 cents per sample
Lambda project
- Looked for part avaliability, explored lambda genome

IGEM:PennState/Labbook/GalenLynch/2007-6-20