IGEM:PennState/Labbook/GalenLynch: Difference between revisions
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name=iGEM:PennState/Labbook/GalenLynch | name=iGEM:PennState/Labbook/GalenLynch | ||
date=2007/10/01 | date=2007/10/01 | ||
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name=iGEM:PennState/Labbook/GalenLynch | |||
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<h2 | <h2>April 30th, 2007: Project Proposal Presentations</h2> | ||
[<span class="_togglegroup _toggle _toggler">show</span><span class="_toggle _toggler" style="display:none;">hide</span>] | |||
<div class="_toggle" style="display:none;"> | <div class="_toggle" style="display:none;"> | ||
<h3>Bacterial Magnetosomes</h3> | |||
<table|left> | <table|left> | ||
<tr> | <tr> | ||
Line 57: | Line 65: | ||
</tr> | </tr> | ||
</table> | </table> | ||
<h3>Biogenic Nanotubes</h3> | |||
<h3 | |||
<font face ="Tahoma"> | <font face ="Tahoma"> | ||
Disclaimer: It turns out this presentation was looking under the wrong name, | Disclaimer: It turns out this presentation was looking under the wrong name, | ||
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</tr> | </tr> | ||
</table> | </table> | ||
</div> | </div> | ||
<h2>[<span class="_togglegroup _toggle _toggler"> | <h2>May 30th, 2007: Project Proposal Presentation</h2> | ||
[<span class="_togglegroup _toggle _toggler">show</span><span class="_toggle _toggler" style="display:none;">hide</span>] | |||
<div class="_toggle" style="display:none;"> | <div class="_toggle" style="display:none;"> | ||
<h3>'''Bio-Dosimetry'''</h3> | |||
<div class="_toggle" style="display:none;"> | <div class="_toggle" style="display:none;"> | ||
<table|left> | <table|left> | ||
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</table> | </table> | ||
</div> | </div> | ||
</div> | |||
<h2>June 4th, 2007: Lab Meeting Presentation</h2> | |||
[<span class="_togglegroup _toggle _toggler">show</span><span class="_toggle _toggler" style="display:none;">hide</span>] | |||
<div class="_toggle" style="display:none;"> | |||
<h3>'''iGEM Meeting'''</h3> | |||
<table|left> | |||
<tr> | |||
<td>[[Image:psuigemnrj5011june041.jpg|thumb|]]</td> | |||
<td>[[Image:psuigemnrj5011june042.jpg|thumb|]]</td> | |||
<td>[[Image:psuigemnrj5011june043.jpg|thumb|]]</td> | |||
</tr> | |||
<tr> | |||
<td>[[Image:psuigemnrj5011june044.jpg|thumb|]]</td> | |||
<td>[[Image:psuigemnrj5011june045.jpg|thumb|]]</td> | |||
<td>[[Image:psuigemnrj5011june046.jpg|thumb|]]</td> | |||
</tr> | |||
<tr> | |||
<td>[[Image:psuigemnrj5011june047.jpg|thumb|]]</td> | |||
<td>[[Image:psuigemnrj5011june048.jpg|thumb|]]</td> | |||
</tr> | |||
</table> | |||
</div> | |||
<h2>June 13th, 2007: XylR Biobricks and Primers</h2> | |||
[<span class="_togglegroup _toggle _toggler">show</span><span class="_toggle _toggler" style="display:none;">hide</span>] | |||
<div class="_toggle" style="display:none;"> | |||
Note: Start codons are in green, stop codons are in read, translational terminators are in blue. | |||
All sequences are shown without biobrick restriction sequences | |||
<h3>XylR with promoter, rbs and terminator (Forward)</h3> | |||
<html><pre>GCAACCAAAC GCCGTTCTTG ATTTTGATAA AAATTTTCTC AAAGCCGGTT ACGTATTACC | |||
GGTTTTGAGT TTTTGCATGA TTCAGCAGGA AAAGAACC<font color=green>AT G</font>TTTACTAAA CGTCACCGCA | |||
TCACATTACT GTTCAATGCC AATAAAGCCT ATGACCGGCA GGTAGTAGAA GGCGTAGGGG | |||
AATATTTACA GGCGTCACAA TCGGAATGGG ATATTTTCAT TGAAGAAGAT TTCCGCGCCC | |||
GCATTGATAA AATCAAGGAC TGGTTAGGAG ATGGCGTCAT TGCCGACTTC GACGACAAAC | |||
AGATCGAGCA AGCGCTGGCT GATGTCGACG TCCCCATTGT TGGGGTTGGC GGCTCGTATC | |||
ACCTTGCAGA AAGTTACCCA CCCGTTCATT ACATTGCCAC CGATAACTAT GCGCTGGTTG | |||
AAAGCGCATT TTTGCATTTA AAAGAGAAAG GCGTTAACCG CTTTGCTTTT TATGGTCTTC | |||
CGGAATCAAG CGGCAAACGT TGGGCCACTG AGCGCGAATA TGCATTTCGT CAGCTTGTCG | |||
CCGAAGAAAA GTATCGCGGA GTGGTTTATC AGGGGTTAGA AACCGCGCCA GAGAACTGGC | |||
AACACGCGCA AAATCGGCTG GCAGACTGGC TACAAACGCT ACCACCGCAA ACCGGGATTA | |||
TTGCCGTTAC TGACGCCCGA GCGCGGCATA TTCTGCAAGT ATGTGAACAT CTACATATTC | |||
CCGTACCGGA AAAATTATGC GTGATTGGCA TCGATAACGA AGAACTGACC CGCTATCTGT | |||
CGCGTGTCGC CCTTTCTTCG GTCGCTCAGG GCGCGCGGCA AATGGGCTAT CAGGCGGCAA | |||
AACTGTTGCA TCGATTATTA GATAAAGAAG AAATGCCGCT ACAGCGAATT TTGGTCCCAC | |||
CAGTTCGCGT CATTGAACGG CGCTCAACAG ATTATCGCTC GCTGACCGAT CCCGCCGTTA | |||
TTCAGGCCAT GCATTACATT CGTAATCACG CCTGTAAAGG GATTAAAGTG GATCAGGTAC | |||
TGGATGCGGT CGGGATCTCG CGCTCCAATC TTGAGAAGCG TTTTAAAGAA GAGGTGGGTG | |||
AAACCATCCA TGCCATGATT CATGCCGAGA AGCTGGAGAA AGCGCGCAGT CTGCTGATTT | |||
CAACCACCTT GTCGATCAAT GAGATATCGC AAATGTGCGG TTATCCATCG CTGCAATATT | |||
TCTACTCTGT TTTTAAAAAA GCATATGACA CGACGCCAAA AGAGTATCGC GATGTAAATA | |||
GCGAGGTCAT GTTG<font color = red>TAG</font>CGA TGATGAGAAT TGTCGGCGTC ACATCAGGTA ACGCTGCGTG | |||
GTTGTCGGAT GCGGCGTGAA CGCCTTATCC GACCTACCCG CCAGGCATGA TAAAACGCAC | |||
CAACAACGCT TCAGGCTCGT AGCTCAACTG CCTGAGACAA AGTAAAA<font color=blue>AGC CTTATCCGAC | |||
TGACAAGTCG GATAAGGCT</font>C AAGGAAATGC AAT</pre></html> | |||
<h4>Primers:</h4> | |||
ggaattcgcg gccgcttcta gag GCAACCAAAC GCCGTTCT (left side) | |||
Sequence for operations: ggaattcgcggccgcttctagagGCAACCAAACGCCGTTCT | |||
*TmC 63,81 | |||
ctgcagcggc cgctactagt a ATTGCATTTC CTTGAGCCTT ATCC (right side) | |||
Sequence for operations: ctgcagcggccgctactagtaATTGCATTTCCTTGAGCCTTATCC | |||
*TmC 63,78 | |||
<h3>XylR with promoter, rbs and terminator (Reverse)</h3> | |||
<html><pre>ATTGCATTTC CTTG<font color=blue>AGCCTT ATCCGACTTG TCAGTCGGAT AAGGCT</font>TTTT ACTTTGTCTC | |||
AGGCAGTTGA GCTACGAGCC TGAAGCGTTG TTGGTGCGTT TTATCATGCC TGGCGGGTAG | |||
GTCGGATAAG GCGTTCACGC CGCATCCGAC AACCACGCAG CGTTACCTGA TGTGACGCCG | |||
ACAATTCTCA TCATCG<font color=red>CTA</font>C AACATGACCT CGCTATTTAC ATCGCGATAC TCTTTTGGCG | |||
TCGTGTCATA TGCTTTTTTA AAAACAGAGT AGAAATATTG CAGCGATGGA TAACCGCACA | |||
TTTGCGATAT CTCATTGATC GACAAGGTGG TTGAAATCAG CAGACTGCGC GCTTTCTCCA | |||
GCTTCTCGGC ATGAATCATG GCATGGATGG TTTCACCCAC CTCTTCTTTA AAACGCTTCT | |||
CAAGATTGGA GCGCGAGATC CCGACCGCAT CCAGTACCTG ATCCACTTTA ATCCCTTTAC | |||
AGGCGTGATT ACGAATGTAA TGCATGGCCT GAATAACGGC GGGATCGGTC AGCGAGCGAT | |||
AATCTGTTGA GCGCCGTTCA ATGACGCGAA CTGGTGGGAC CAAAATTCGC TGTAGCGGCA | |||
TTTCTTCTTT ATCTAATAAT CGATGCAACA GTTTTGCCGC CTGATAGCCC ATTTGCCGCG | |||
CGCCCTGAGC GACCGAAGAA AGGGCGACAC GCGACAGATA GCGGGTCAGT TCTTCGTTAT | |||
CGATGCCAAT CACGCATAAT TTTTCCGGTA CGGGAATATG TAGATGTTCA CATACTTGCA | |||
GAATATGCCG CGCTCGGGCG TCAGTAACGG CAATAATCCC GGTTTGCGGT GGTAGCGTTT | |||
GTAGCCAGTC TGCCAGCCGA TTTTGCGCGT GTTGCCAGTT CTCTGGCGCG GTTTCTAACC | |||
CCTGATAAAC CACTCCGCGA TACTTTTCTT CGGCGACAAG CTGACGAAAT GCATATTCGC | |||
GCTCAGTGGC CCAACGTTTG CCGCTTGATT CCGGAAGACC ATAAAAAGCA AAGCGGTTAA | |||
CGCCTTTCTC TTTTAAATGC AAAAATGCGC TTTCAACCAG CGCATAGTTA TCGGTGGCAA | |||
TGTAATGAAC GGGTGGGTAA CTTTCTGCAA GGTGATACGA GCCGCCAACC CCAACAATGG | |||
GGACGTCGAC ATCAGCCAGC GCTTGCTCGA TCTGTTTGTC GTCGAAGTCG GCAATGACGC | |||
CATCTCCTAA CCAGTCCTTG ATTTTATCAA TGCGGGCGCG GAAATCTTCT TCAATGAAAA | |||
TATCCCATTC CGATTGTGAC GCCTGTAAAT ATTCCCCTAC GCCTTCTACT ACCTGCCGGT | |||
CATAGGCTTT ATTGGCATTG AACAGTAATG TGATGCGGTG ACGTTTAGTA AA<font color<font color=green>CAT</font>GGTTC | |||
TTTTCCTGCT GAATCATGCA AAAACTCAAA ACCGGTAATA CGTAACCGGC TTTGAGAAAA | |||
TTTTTATCAA AATCAAGAAC GGCGTTTGGT TGC</pre></html> | |||
<h4>Primers:</h4> | |||
ggaattcgcg gccgcttcta gag ATTGCATTTC CTTGAGCCTT ATCC (right side) | |||
Sequence for operations: ggaattcgcggccgcttctagagATTGCATTTCCTTGAGCCTTATCC | |||
*TmC 63,79 | |||
ctgcagcggc cgctactagt a GCAACCAAAC GCCGTTCT (left side) | |||
Sequence for operations: ctgcagcggccgctactagtaGCAACCAAACGCCGTTCT | |||
*TmC 63,80 | |||
<h3>XylR open reading frame (Forward)</h3> | |||
<html><pre><font color=green>ATG</font>TTTACTA AACGTCACCG CATCACATTA CTGTTCAATG CCAATAAAGC CTATGACCGG | |||
CAGGTAGTAG AAGGCGTAGG GGAATATTTA CAGGCGTCAC AATCGGAATG GGATATTTTC | |||
ATTGAAGAAG ATTTCCGCGC CCGCATTGAT AAAATCAAGG ACTGGTTAGG AGATGGCGTC | |||
ATTGCCGACT TCGACGACAA ACAGATCGAG CAAGCGCTGG CTGATGTCGA CGTCCCCATT | |||
GTTGGGGTTG GCGGCTCGTA TCACCTTGCA GAAAGTTACC CACCCGTTCA TTACATTGCC | |||
ACCGATAACT ATGCGCTGGT TGAAAGCGCA TTTTTGCATT TAAAAGAGAA AGGCGTTAAC | |||
CGCTTTGCTT TTTATGGTCT TCCGGAATCA AGCGGCAAAC GTTGGGCCAC TGAGCGCGAA | |||
TATGCATTTC GTCAGCTTGT CGCCGAAGAA AAGTATCGCG GAGTGGTTTA TCAGGGGTTA | |||
GAAACCGCGC CAGAGAACTG GCAACACGCG CAAAATCGGC TGGCAGACTG GCTACAAACG | |||
CTACCACCGC AAACCGGGAT TATTGCCGTT ACTGACGCCC GAGCGCGGCA TATTCTGCAA | |||
GTATGTGAAC ATCTACATAT TCCCGTACCG GAAAAATTAT GCGTGATTGG CATCGATAAC | |||
GAAGAACTGA CCCGCTATCT GTCGCGTGTC GCCCTTTCTT CGGTCGCTCA GGGCGCGCGG | |||
CAAATGGGCT ATCAGGCGGC AAAACTGTTG CATCGATTAT TAGATAAAGA AGAAATGCCG | |||
CTACAGCGAA TTTTGGTCCC ACCAGTTCGC GTCATTGAAC GGCGCTCAAC AGATTATCGC | |||
TCGCTGACCG ATCCCGCCGT TATTCAGGCC ATGCATTACA TTCGTAATCA CGCCTGTAAA | |||
GGGATTAAAG TGGATCAGGT ACTGGATGCG GTCGGGATCT CGCGCTCCAA TCTTGAGAAG | |||
CGTTTTAAAG AAGAGGTGGG TGAAACCATC CATGCCATGA TTCATGCCGA GAAGCTGGAG | |||
AAAGCGCGCA GTCTGCTGAT TTCAACCACC TTGTCGATCA ATGAGATATC GCAAATGTGC | |||
GGTTATCCAT CGCTGCAATA TTTCTACTCT GTTTTTAAAA AAGCATATGA CACGACGCCA | |||
AAAGAGTATC GCGATGTAAA TAGCGAGGTC ATGTTG<font color=red>TAG</font></pre></html> | |||
<h4>Primers:</h4> | |||
ggaattcgcg gccgcttcta gag ATGTTTACTA AACGTCACCG CATCA left side | |||
Sequence without spaces: ggaattcgcggccgcttctagagATGTTTACTAAACGTCACCGCATCA | |||
*TmC 65,79 | |||
ctgcagcggc cgctactagt a CTACAACATG ACCTCGCTAT TTACAT right side | |||
Sequence wihout spaces: ctgcagcggccgctactagtaCTACAACATGACCTCGCTATTTACAT | |||
*TmC 62,77 | |||
<h3>XylR open reading frame (Reverse)</h3> | |||
<html><pre><font color=red>CTA</font>CAACATG ACCTCGCTAT TTACATCGCG ATACTCTTTT GGCGTCGTGT CATATGCTTT | |||
TTTAAAAACA GAGTAGAAAT ATTGCAGCGA TGGATAACCG CACATTTGCG ATATCTCATT | |||
GATCGACAAG GTGGTTGAAA TCAGCAGACT GCGCGCTTTC TCCAGCTTCT CGGCATGAAT | |||
CATGGCATGG ATGGTTTCAC CCACCTCTTC TTTAAAACGC TTCTCAAGAT TGGAGCGCGA | |||
GATCCCGACC GCATCCAGTA CCTGATCCAC TTTAATCCCT TTACAGGCGT GATTACGAAT | |||
GTAATGCATG GCCTGAATAA CGGCGGGATC GGTCAGCGAG CGATAATCTG TTGAGCGCCG | |||
TTCAATGACG CGAACTGGTG GGACCAAAAT TCGCTGTAGC GGCATTTCTT CTTTATCTAA | |||
TAATCGATGC AACAGTTTTG CCGCCTGATA GCCCATTTGC CGCGCGCCCT GAGCGACCGA | |||
AGAAAGGGCG ACACGCGACA GATAGCGGGT CAGTTCTTCG TTATCGATGC CAATCACGCA | |||
TAATTTTTCC GGTACGGGAA TATGTAGATG TTCACATACT TGCAGAATAT GCCGCGCTCG | |||
GGCGTCAGTA ACGGCAATAA TCCCGGTTTG CGGTGGTAGC GTTTGTAGCC AGTCTGCCAG | |||
CCGATTTTGC GCGTGTTGCC AGTTCTCTGG CGCGGTTTCT AACCCCTGAT AAACCACTCC | |||
GCGATACTTT TCTTCGGCGA CAAGCTGACG AAATGCATAT TCGCGCTCAG TGGCCCAACG | |||
TTTGCCGCTT GATTCCGGAA GACCATAAAA AGCAAAGCGG TTAACGCCTT TCTCTTTTAA | |||
ATGCAAAAAT GCGCTTTCAA CCAGCGCATA GTTATCGGTG GCAATGTAAT GAACGGGTGG | |||
GTAACTTTCT GCAAGGTGAT ACGAGCCGCC AACCCCAACA ATGGGGACGT CGACATCAGC | |||
CAGCGCTTGC TCGATCTGTT TGTCGTCGAA GTCGGCAATG ACGCCATCTC CTAACCAGTC | |||
CTTGATTTTA TCAATGCGGG CGCGGAAATC TTCTTCAATG AAAATATCCC ATTCCGATTG | |||
TGACGCCTGT AAATATTCCC CTACGCCTTC TACTACCTGC CGGTCATAGG CTTTATTGGC | |||
ATTGAACAGT AATGTGATGC GGTGACGTTT AGTAAA<font color=green>CAT</font></pre></html> | |||
<h4>Primers:</h4> | |||
ggaattcgcg gccgcttcta gag CTACAACATG ACCTCGCTAT TTACAT left side | |||
Sequence for operations: ggaattcgcggccgcttctagagCTACAACATGACCTCGCTATTTACAT | |||
*TmC 62,78 | |||
ctgcagcggc cgctactagt a ATGTTTACTA AACGTCACCG CATCA right side | |||
Sequence for operations: ctgcagcggccgctactagtaATGTTTACTAAACGTCACCGCATCA | |||
*TmC 65,78 | |||
</div> | |||
<h2> June 28th, 2007: Lambda Biobricks and Primers</h2> | |||
[<span class="_togglegroup _toggle _toggler">show</span><span class="_toggle _toggler" style="display:none;">hide</span>] | |||
<div class="_toggle" style="display:none;"> | |||
<h3>Lambda control region (O<sub>R</sub>)</h3> | |||
*Contains O<sub>R</sub>1, O<sub>R</sub>2, and O<sub>R</sub>3, shown in reverse order (i.e. the leftmost binding region below is O<sub>R</sub>3) | |||
*Bi directional promoter | |||
**in the lambda phage, CI is to the left(P<sub>RM</sub>), cro is to the right(P<sub>R</sub>) | |||
*recognized by cro, CI | |||
**cro binding affinities: O<sub>R</sub>3, O<sub>R</sub>2 ~ O<sub>R</sub>1 (O<sub>R</sub>1 is slightly less) | |||
**CI binding affinites: O<sub>R</sub>1, O<sub>R</sub>2 ~ O<sub>R</sub>3 (O<sub>R</sub>3 is slightly less) | |||
*CI acts as a positive regulator for P<sub>RM</sub> when bound to O<sub>R</sub>2, but and a negative regulator for P<sub>R</sub> when bound to O<sub>R</sub>3 | |||
<html><pre>ACGTTA<font color=cyan>AATC </font><font color= blue>TA</font><font color=green>TCACCGCA AGGGATA</font>AA<font color=cyan>T ATC</font><font color = blue>TA</font><font color = green>ACACC GTGCGTG</font><font color =blue>TTG</font><font color=cyan> ACT</font>ATTT<font color=green>TAC | |||
CTCTGGCGGT </font><font color=blue>GATA</font><font color=cyan>AT</font>GGTT GC</pre></html> | |||
Cro and CI binding regions are depicted in <font color = green>green</font>, -10 and -35 sequences in <font color = cyan>cyan</font>, and shared regions are in <font color = blue>blue</font> | |||
<h4>Primers</h4> | |||
Readable primer:ggaattcgcg gccgcttcta gag ACGTTAAATC TATCACCGC (left side) | |||
Primer sequence:ggaattcgcggccgcttctagagACGTTAAATCTATCACCGC | |||
*Mp: 57,77 | |||
Readable primer:ctgcagcggc cgctactagt a GCAACCATTA TCACCGC (right side) | |||
Primer sequence:ctgcagcggccgctactagtaGCAACCATTATCACCGC | |||
*Mp: 58,78 | |||
<h3>Lambda Repressor (CI)</h3> | |||
*Forms dimers | |||
**Dimers associate to form tetramers, octamers etc | |||
*Binds to O<sub>L</sub> and O<sub>R</sub> operators in bacteriophage genome | |||
*Cleaved by activated RecA protein (activates with ssDNA exposure) | |||
<html><pre><font color=green>ATG</font>AGCACAA AAAAGAAACC ATTAACACAA GAGCAGCTTG AGGACGCACG TCGCCTTAAA | |||
GCAATTTATG AAAAAAAGAA AAATGAACTT GGCTTATCCC AGGAATCTGT CGCAGACAAG | |||
ATGGGGATGG GGCAGTCAGG CGTTGGTGCT TTATTTAATG GCATCAATGC ATTAAATGCT | |||
TATAACGCCG CATTGCTTGC AAAAATTCTC AAAGTTAGCG TTGAAGAATT TAGCCCTTCA | |||
ATCGCCAGAG AAATCTACGA GATGTATGAA GCGGTTAGTA TGCAGCCGTC ACTTAGAAGT | |||
GAGTATGAGT ACCCTGTTTT TTCTCATGTT CAGGCAGGGA TGTTCTCACC TGAGCTTAGA | |||
ACCTTTACCA AAGGTGATGC GGAGAGATGG GTAAGCACAA CCAAAAAAGC CAGTGATTCT | |||
GCATTCTGGC TTGAGGTTGA AGGTAATTCC ATGACCGCAC CAACAGGCTC CAAGCCAAGC | |||
TTTCCTGACG GAATGTTAAT TCTCGTTGAC CCTGAGCAGG CTGTTGAGCC AGGTGATTTC | |||
TGCATAGCCA GACTTGGGGG TGATGAGTTT ACCTTCAAGA AACTGATCAG GGATAGCGGT | |||
CAGGTGTTTT TACAACCACT AAACCCACAG TACCCAATGA TCCCATGCAA TGAGAGTTGT | |||
TCCGTTGTGG GGAAAGTTAT CGCTAGTCAG TGGCCTGAAG AGACGTTTGG C<font color=red>TGA</font></pre></html> | |||
Start codon is in <font color=green>green</font> and stop codon is in <font color=red>red</font> | |||
<h4>Primers</h4> | |||
Readable Primer:ggaattcgcg gccgcttcta gag ATGAGCACAA AAAAGAAACC (left side) | |||
Primer sequence:ggaattcgcggccgcttctagagATGAGCACAAAAAAGAAACC | |||
*Mp: 56,77 | |||
Readable primer:ctgcagcggc cgctactagt a TCAGCCAAAC GTCTCTTC (right side) | |||
Primer sequence:ctgcagcggccgctactagtaTCAGCCAAACGTCTCTTC | |||
*Mp: 58,78 | |||
<h3>Lambda Repressor (CI) with degredation tag (LVA)</h3> | |||
Part [http://parts.mit.edu/registry/index.php/Part:BBa_C0051 BBa_C0051] on MIT's parts registry | |||
*same as CI repressor, but with degredation tag on the N terminus to promote degredation | |||
<h3>Lambda AntiRepressor (Cro)</h3> | |||
*Binds to O<sub>R</sub> and O<sub>L</sub> operators | |||
*transcriptional activator, repressor | |||
*favors lytic pathway in lambda bacteriophage | |||
<html><pre><font color=green>ATG</font>GAACAAC GCATAACCCT GAAAGATTAT GCAATGCGCT TTGGGCAAAC CAAGACAGCT | |||
AAAGATCTCG GCGTATATCA AAGCGCGATC AACAAGGCCA TTCATGCAGG CCGAAAGATT | |||
TTTTTAACTA TAAACGCTGA TGGAAGCGTT TATGCGGAAG AGGTAAAGCC CTTCCCGAGT | |||
AACAAAAAAA CAACAGCA<font color=red>TA A</font></pre></html> | |||
Start codon is in <font color=green>green</font> and stop codon is in <font color=red>red</font> | |||
<h4>Primers</h4> | |||
Readable primer:ggaattcgcg gccgcttcta gag ATGGAACAAC GCATAACC (left side) | |||
Primer sequence:ggaattcgcggccgcttctagagATGGAACAACGCATAACC | |||
*Mp: 57,78 | |||
Readable primer:ctgcagcggc cgctactagt a TTATGCTGTT GTTTTTTTGT TACT (right side) | |||
Primer sequence:ctgcagcggccgctactagtaTTATGCTGTTGTTTTTTTGTTACT | |||
*Mp: 58,75 | |||
<h2>Oligo Order results:</h2> | |||
*Primer 260 LBOrL 75.48 nm, 1046.19 ug, 100nM | |||
*Primer 261 LBOrR 79.09 nm, 991.83 ug, 100nM | |||
*Primer 262 LBClL 73.48 nm, 1042.71 ug, 100nM | |||
*Primer 263 LBClR 79.76 nm, 1026.56 ug, 100nM | |||
*Primer 264 LBCroL 79.42nm, 1074.55 ug, 100nM | |||
*Primer 265 LBCroR 81.02 nm, 1206.56 ug, 100nM | |||
</div> | |||
<h2>June 30th, 2007: Competency Transformations</h2> | |||
[<span class="_togglegroup _toggle _toggler">show</span><span class="_toggle _toggler" style="display:none;">hide</span>] | |||
<div class="_toggle" style="display:none;"> | |||
<table|left> | |||
<tr> | |||
<td>'''Transformations of frozen competent cells'''</td> | |||
</tr> | |||
<tr> | |||
<td>[[Image:GFL DB 31 puc 6 30.png|thumb|DB3.1 with puc19]]</td> | |||
<td>[[Image:GFL DB 31 neg 6 30.png|thumb|DB3.1 negative control]]</td> | |||
<td>[[Image:GFL DH5a puc 6 30.png|thumb|DH5alpha with puc19]]</td> | |||
</tr> | |||
<tr> | |||
<td>[[Image:GFL DH5a puc close 6 30.png|thumb|DH5alpha with puc19 closeup]]</td> | |||
<td>[[Image:GFL DH5a neg 6 30.png|thumb|DH5alpha negative control]]</td> | |||
</tr> | |||
</table> | |||
Note that all images above have had their contrast levels adjusted in photoshop to highlight colonies, for originals contact [[user:tachyon60|me]]. | |||
</div> | </div> |
Latest revision as of 12:30, 24 December 2007
Galen Lynch Lab Notebook
- Penn State iGEM 2007
- Penn State Undergraduate
- Email gfl110@psu.edu
Calender
[show ]
April 30th, 2007: Project Proposal Presentations
[show ]
May 30th, 2007: Project Proposal Presentation
[show ]
June 4th, 2007: Lab Meeting Presentation
[show ]
June 13th, 2007: XylR Biobricks and Primers
[show ]
June 28th, 2007: Lambda Biobricks and Primers
[show ]
June 30th, 2007: Competency Transformations
[show ]