IGEM:Peking University/2008/Notebook/Group 2/2008/07/20: Difference between revisions

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(Autocreate 2008/07/20 Entry for IGEM:Peking_University/2008/Notebook/Group_2)
 
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==Entry title==
==PKU_080720_G2_1==
* Insert your content here.
 
<hr class=divider>
 
 
::'''Plasmid Miniprep-T-Gal4<sub>wt</sub>-4/T-Gal4<sub>m1278</sub>-12/T-Gal4<sub>m1743</sub>-12'''
 
 
::'''Enzyme cutting-checking-T-Gal4<sub>wt</sub>/T-Gal4<sub>m1278</sub>/T-Gal4<sub>m1743</sub>'''
 
{|border="1" cellspacing="0" align="center"
|+
|-
|gal4(WT)-EcoRI-HindIII||gal4(1278-1743)-EcoRI-HindIII(1)||gal4(1278-1743)-EcoRI-HindIII(2)
|-
|[[Image:PKU-7-20_gal4(WT)-EcoRI-HindIII.tif|200px]]||[[Image:PKU-7-20_gal4(1278-1743)-EcoRI-HindIII(2).tif|200px]]||[[Image:PKU-7-20_gal4(1278-1743)-EcoRI-HindIII(2).tif|200px]]
|-
|}
 
 
 
{|border="1" cellspacing="0" align="center"
|+
|-
| ||Volume (μL)
|-
| plasmid||5
|-
|EcoRI||0.3||×30
|-
|HindIII||0.3
|-
|M Buffer||2
|-
|ddH<sub>2</sub>O||12.4
|}
 




Revision as of 12:35, 22 July 2008

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PKU_080720_G2_1


Plasmid Miniprep-T-Gal4wt-4/T-Gal4m1278-12/T-Gal4m1743-12


Enzyme cutting-checking-T-Gal4wt/T-Gal4m1278/T-Gal4m1743
gal4(WT)-EcoRI-HindIII gal4(1278-1743)-EcoRI-HindIII(1) gal4(1278-1743)-EcoRI-HindIII(2)


Volume (μL)
plasmid 5
EcoRI 0.3 ×30
HindIII 0.3
M Buffer 2
ddH2O 12.4




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