IGEM:Peking University/2008/Notebook/Group 2/2008/06/27: Difference between revisions

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(Autocreate 2008/06/27 Entry for IGEM:Peking_University/2008/Notebook/Group_2)
 
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==Entry title==
==0627==
* Insert your content here.
{|border="1" cellspacing="0"
|+ '''TITLE: PCR-pPT-Gal4-Transtart  20ul*6'''
|-
| ||Volume (μL)
|-
|Template||0.5
|-
|dNTP||2
|-
|Enzyme||0.5
|-
|Buffer||2
|-
|F-Primer||0.2
|-
|R-Primer||0.2
|-
|ddH<sub>2O</sub>||14
|}
 
::'''conditions'''
{|border="1" cellspacing="0"
|+
|-
|Temperature||Time Length(0:00:00)
|-
| 94°C||02:00
|-
| 94°C||01:00||4 cycles
|-
| 50°C||02:00||4 cycles
|-
| 72°C||05:00||4 cycles
|-
| 94°C||01:00||30 cycles
|-
| 55.2,55.6,57.6,59.5,61.3,63.9°C||01:00||30 cycles
|-
| 72°C||03:00||30 cycles
|-
| 72°C||10:00
|-
| 4°C||Hold
|}
<hr class=divider>
::'''Enzyme cutting-T-Gal4-XbaI-20*6 μL'''
 
{|border="1" cellspacing="0"
|+
|-
| ||Volumn(μL)
|-
| DNA||2
|-
| XbaI||1
|-
| M Buffer||2
|-
| BSA||2
|-
|ddH<sub>2</sub>O||13
|}
<hr class=divider>
::'''Enzyme cutting-T-Gal4-SpeI-HindIII-20*4 μL'''
 
{|border="1" cellspacing="0"
|+
|-
| ||Volumn(μL)
|-
| DNA||2
|-
| SpeI||1
|-
| HindIII||1
|-
| M Buffer||2
|-
|ddH<sub>2</sub>O||14
|}




Revision as of 12:27, 22 July 2008

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0627

TITLE: PCR-pPT-Gal4-Transtart 20ul*6
Volume (μL)
Template 0.5
dNTP 2
Enzyme 0.5
Buffer 2
F-Primer 0.2
R-Primer 0.2
ddH2O 14
conditions
Temperature Time Length(0:00:00)
94°C 02:00
94°C 01:00 4 cycles
50°C 02:00 4 cycles
72°C 05:00 4 cycles
94°C 01:00 30 cycles
55.2,55.6,57.6,59.5,61.3,63.9°C 01:00 30 cycles
72°C 03:00 30 cycles
72°C 10:00
4°C Hold
Enzyme cutting-T-Gal4-XbaI-20*6 μL
Volumn(μL)
DNA 2
XbaI 1
M Buffer 2
BSA 2
ddH2O 13
Enzyme cutting-T-Gal4-SpeI-HindIII-20*4 μL
Volumn(μL)
DNA 2
SpeI 1
HindIII 1
M Buffer 2
ddH2O 14



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