IGEM:Peking University/2008/Notebook/Group 2/2008/05/28: Difference between revisions
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(Autocreate 2008/05/28 Entry for IGEM:Peking_University/2008/Notebook/Group_2) |
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| colspan="2"| | | colspan="2"| | ||
== | ==PCR== | ||
{|border="1" cellspacing="0" | |||
|+ '''TITLE: PCR-Budding Yeast Genome-Gal4-K.O.D 50ul''' | |||
|- | |||
| ||Volume (μL) | |||
|- | |||
|Template (Budding Yeast Genome)||1 | |||
|- | |||
|dNTP||5 | |||
|- | |||
|Enzyme||1 | |||
|- | |||
|Buffer||5 | |||
|- | |||
|MgSO4(For KOD)||2 | |||
|- | |||
|F-Gal4-HindIII-ApaI ||1 | |||
|- | |||
|R-Gal4-EcoRI-Xhol||1 | |||
|- | |||
|ddH2O||34 | |||
|} | |||
::'''conditions''' | |||
{|border="1" cellspacing="0" | |||
|+ | |||
|- | |||
|Temperature||Time Length(0:00:00) | |||
|- | |||
| 94°C||02:00 | |||
|- | |||
| 94°C||00:20||30 cycles | |||
|- | |||
| 59°C||00:30||30 cycles | |||
|- | |||
| 55°C||00:30||30 cycles | |||
|- | |||
| 68°C||03:00||30 cycles | |||
|- | |||
| 68°C||07:00 | |||
|- | |||
| 4°C||Hold | |||
|} | |||
'''Failed.''' | |||
Revision as of 12:15, 22 July 2008
Group 2 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||
PCR
Failed.
|