IGEM:Peking University/2008/Notebook/Group 1/2008/05/19: Difference between revisions

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(Autocreate 2008/05/19 Entry for IGEM:Peking_University/2008/Notebook/Group_1)
 
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==Entry title==
==PKU_080519_G1_1==
* Insert your content here.
<hr class=divider>
::'''Cultivation- E.Coli(liguid-Amp<sup>+</sup>)'''
 
<hr class=divider>
::'''Plasmid Miniprep-pGREG504/pADH'''
 
<hr class=divider>
::'''Enzyme cutting-pGREG504/pADH-EcoRV'''
{|
[[Image:PKU_080519_check-pGREG504-pADH.tif|600px]]
|}
{|border="1" cellspacing="0"
|+
|-
| ||Volume (μL)
|-
| pGREG504/pADH||5
|-
|EcoRV||0.2||*13
|-
|H Buffer||2
|-
|ddH<sub>2</sub>O||12.8
|}
 
 
::'''Enzyme cutting-pGREG504/pADH-SacI-BamHI'''
{|border="1" cellspacing="0"
|+
|-
| ||Volume (μL)
|-
| pGREG504/pADH||5
|-
|SacI||0.2||*13
|-
|BamHI||0.2||
|-
|K Buffer||2
|-
|ddH<sub>2</sub>O||13.6
|}
 




Revision as of 08:25, 18 July 2008

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PKU_080519_G1_1

Cultivation- E.Coli(liguid-Amp+)
Plasmid Miniprep-pGREG504/pADH
Enzyme cutting-pGREG504/pADH-EcoRV
Volume (μL)
pGREG504/pADH 5
EcoRV 0.2 *13
H Buffer 2
ddH2O 12.8


Enzyme cutting-pGREG504/pADH-SacI-BamHI
Volume (μL)
pGREG504/pADH 5
SacI 0.2 *13
BamHI 0.2
K Buffer 2
ddH2O 13.6




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