IGEM:Peking University/2008/Notebook/Group 1/2008/05/13: Difference between revisions
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(Autocreate 2008/05/13 Entry for IGEM:Peking_University/2008/Notebook/Group_1) |
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== | ==PKU_080513_G1_1== | ||
* | <hr class=divider> | ||
::'''PCR- pGREG505/(TEF-lacI-ADHlacS-GFP)-adhlac-50ul''' | |||
{|align="right" | |||
[[Image:PKU_080513_ADH-PCR-check.tif|400px]] | |||
|} | |||
{|border="1" cellspacing="0" | |||
|+ | |||
|- | |||
| ||Volume (μL) | |||
|- | |||
| pGREG505/(TEF-lacI-ADHlacS-GFP)||1 | |||
|- | |||
|dNTP||5 | |||
|- | |||
|transtart taq||1 | |||
|- | |||
|transtart Buffer||5 | |||
|- | |||
|F-Primer||1 | |||
|- | |||
|R-Primer||1 | |||
|- | |||
|ddH<sub>2</sub>O||36 | |||
|} | |||
::'''conditions''' | |||
{|border="1" cellspacing="0" | |||
|+ | |||
|- | |||
|Temperature(°C)||Time Length(0:00:00) | |||
|- | |||
| 94||05:00 | |||
|- | |||
| 94||00:30||30 cycles | |||
|- | |||
| 50,55,60||00:30||30 cycles | |||
|- | |||
| 72||01:30||30 cycles | |||
|- | |||
| 72||07:00 | |||
|- | |||
| Alarm||5 times | |||
|- | |||
| Hold||4°C | |||
|} | |||
<hr class=divider> | |||
::'''Enzyme cutting-DNA (pGREG503/504)-SacI-XhoI-20 μL''' | |||
{|align="right" | |||
[[Image:PKU_080513_pGREG504-cut.tif|400px]] | |||
|} | |||
{|border="1" cellspacing="0" | |||
|+ | |||
|- | |||
| ||Volumn(μL) | |||
|- | |||
| pGREG503/504 ||5 | |||
|- | |||
| SacI||0.2 | |||
|- | |||
| XhoI ||0.2||*5 | |||
|- | |||
| M Buffer||2 | |||
|- | |||
|ddH<sub>2</sub>O||12.6 | |||
|} | |||
Revision as of 08:17, 18 July 2008
 Group 1
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