IGEM:Peking University/2008/Notebook/Group 1/2008/05/12: Difference between revisions

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(Autocreate 2008/05/12 Entry for IGEM:Peking_University/2008/Notebook/Group_1)
 
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==Entry title==
==PKU_080512_G1_1==
* Insert your content here.
<hr class=divider>
::'''Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA'''
{|align="right"
[[Image:PKU_080512_ADH-plasmid-check(95min).tif|400px]]
|}


{|border="1" cellspacing="0"
|+
|-
| ||Volume (μL)
|-
|DNA||5
|-
|ClaI||0.2
|-
|BamH1||0.2
|-
|KBuffer||2
|-
|ddH2O ||12.6
|-
|}
<hr class=divider>
::'''PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul'''
{|align="right"
[[Image:PKU_080512_ADH-PCR-check.tif|200px]]
|}
{|border="1" cellspacing="0"
|+
|-
| ||Volume (μL)
|-
| pGREG505/(TEF-lacI-adhlacS-GFP)||5
|-
|dNTP||5
|-
|KOD Plus||1
|-
|KOD Plus Buffer||5
|-
|MgSO<sub>4</sub>||2
|-
|F-Primer||1
|-
|R-Primer||1
|-
|ddH<sub>2</sub>O||34
|}
::'''conditions'''
{|border="1" cellspacing="0"
|+
|-
|Temperature(°C)||Time Length(0:00:00)
|-
| 94||05:00
|-
| 94||00:30||30 cycles
|-
| 55||00:30||30 cycles
|-
| 72||01:30||30 cycles
|-
| 72||07:00
|-
| Alarm||5 times
|-
| Hold||4°C
|}




Revision as of 08:16, 18 July 2008

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PKU_080512_G1_1

Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA
Volume (μL)
DNA 5
ClaI 0.2
BamH1 0.2
KBuffer 2
ddH2O 12.6


PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul
Volume (μL)
pGREG505/(TEF-lacI-adhlacS-GFP) 5
dNTP 5
KOD Plus 1
KOD Plus Buffer 5
MgSO4 2
F-Primer 1
R-Primer 1
ddH2O 34


conditions
Temperature(°C) Time Length(0:00:00)
94 05:00
94 00:30 30 cycles
55 00:30 30 cycles
72 01:30 30 cycles
72 07:00
Alarm 5 times
Hold 4°C



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