IGEM:Peking University/2008/Experiment/PKU 0627 G2 1
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Volume (μL) | |
Template | 0.5 |
dNTP | 2 |
Enzyme | 0.5 |
Buffer | 2 |
F-Primer | 0.2 |
R-Primer | 0.2 |
ddH2O | 14 |
- conditions
Temperature | Time Length(0:00:00) | |
94°C | 02:00 | |
94°C | 01:00 | 4 cycles |
50°C | 02:00 | 4 cycles |
72°C | 05:00 | 4 cycles |
94°C | 01:00 | 30 cycles |
55.2,55.6,57.6,59.5,61.3,63.9°C | 01:00 | 30 cycles |
72°C | 03:00 | 30 cycles |
72°C | 10:00 | |
4°C | Hold |
- Enzyme cutting-T-Gal4-XbaI-20*6 μL
Volumn(μL) | |
DNA | 2 |
XbaI | 1 |
M Buffer | 2 |
BSA | 2 |
ddH2O | 13 |
- Enzyme cutting-T-Gal4-SpeI-HindIII-20*4 μL
Volumn(μL) | |
DNA | 2 |
SpeI | 1 |
HindIII | 1 |
M Buffer | 2 |
ddH2O | 14 |
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