IGEM:Peking University/2008/Experiment/PKU 0607 G2 1: Difference between revisions
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(New page: {|border="1" cellspacing="0" |+ '''TITLE: PCR-Budding Yeast Genome-Gal4-Transtart 50ul*6''' |- | ||Volume (μL) |- |Template （Budding Yeast Genome）||1 |- |dNTP||5 |- |Enzyme||1 |...) |
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Revision as of 05:08, 4 July 2008
| Volume (μL) | |
| Template （Budding Yeast Genome） | 1 |
| dNTP | 5 |
| Enzyme | 1 |
| Buffer | 5 |
| F-Gal4-HindIII-ApaI | 1 |
| R-Gal4-EcoRI-Xhol | 1 |
| ddH2O | 36 |
- conditions
| Temperature | Time Length(0:00:00) | |
| 94°C | 02:00 | |
| 94°C | 01:00 | 4 cycles |
| 50°C | 02:00 | 4 cycles |
| 72°C | 00:30 | 4 cycles |
| 94°C | 01:00 | 30 cycles |
| 49.2,52.6,54.6,56.5,58.2,59.8°C | 02:00 | 30 cycles |
| 72°C | 04:00 | 30 cycles |
| 72°C | 05:00 | |
| 4°C | Hold |
| back to Main Page |
