IGEM:Peking/2007/Switch-Notebook/2007-8-5
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Experimenter: LXL
Precipitate and retract
SDY-EGFP^^(XhoI/SalI)
Ligation
SDY-EGFP pLX007 SDY-EGFP pLX003 pLX007 (self-control) pLX003 (self-control) SDY-EGFP (self-control)
Colony PCR test of ligation product
Col.No. Product -------------------------- 1-46 T1T2-lacZa-pLX003 47,48 lacZa-pLX003(self-ligation-control) 49-76 lacZa-pLX007 78,79 pLX007(self-ligation-control)
8 are incubated.