IGEM:Peking/2007/Switch-Notebook/2007-8-3
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Experimenter: LXL
Transformation of T1TE-pCC010, T1T2-pCC010
Colony PCR test of CD2, RD2, RS3-pMD18-T
Primers: MBF/R
6 of each type are correct and sent for sequencing.
Digestion of fragment and plasmid
2uL pLX007(4) 2uL XhoI 2uL SalI 10uL 10*H buffer 84uL H2O -------------------- 100uL total
2uL SD-lacZa-pLX003 2uL XhoI 2uL AatII 10uL T buffer 10uL BSA 84uL H2O -------------------- 100uL total
22uL T1T2^^(AatII/XhoI) 2uL XhoI 2uL AatII 10uL T buffer 10uL BSA 54uL H2O -------------------- 100uL total