plasmid mini-prep
T1TE-PMD18T
T1T2-pcc010
lacI-pcc009
colony PCR
test pcc009-lacI, no one is right!
colony PCR
test RS1, RS2, RD2, SD1, CD1 with GFP-plx007
some lanes seem to be right, just cultivate them, miniprep and double digestion(XhoI, SalI) tomorrow!
transformation of lacI-pcc009