IGEM:Peking/2007/Switch-Notebook/2007-7-30
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transformation of MCS-pcc009and pcc010
Smear the transformation onto Cm+LB plate
plx007,008,009 positive transformation
Pick colonies
colony PCR of RD2-pGEM-T
using Primer M13F/R
T1TE-pcc010 miniprep
double digestion using KpnI/XhoI and M buffer pcc010 as the negative control
Miniprep of plx007/008/009
using XhoI/SalI to double digest in H buffer
digestion of vector 7-6, 8-6, 9-5
plasmid 2uL
Buffer H 10uL
XhoI/SalI 2uL each
H2O 84uL
total 100uL
fragment lacZa-XS 16uL
buffer H 2uL
SalI 2uL
H2O 70uL
total 100uL