IGEM:Peking/2007/Count:PCR product purifcation
PCR product purifcation
according to Transgen kit protocol
- Take 50-100uL PCR product solution,add 5 times volume solutoin BB,mix, add into spin column,standing for 1 min, 13000rpm for 1 min,discard the flow-through.
- Add 650 mL buffer WB ,13000 rpm for 1min,discard the supernatant
- 13000rpm for 2 min to wipe out the remaining WB
- Put the collection tube into a clean EP tube, Add 50 uL 60C ddH2O in the center of the collection tube,standing for 1 min
- 13000rpm for 1 min.
- store the DNA solution at -20C