IGEM:Peking/2007/Count:Gel-Extraction

quick gel purifcation

according to Transgen kit protocol

1. Agarose gel electrophoresis,cut the DNA band as thin as possible.pute into a clear EP tube.weighing,if the weight is 100mg,the volume can be

consider as 100uL,add solution GSB,put into 55C water bath until complently merge,it takes about 5 minutes

1. cool the tube with water to adjust the tempertature of the solution to room temperature
2. Add the solution into the collection tube, standing for 1 minute,13000rpm for 1 min, discard the flow-through
3. Add 650 uL WB,13000rpm for 1 min,discard the flow-through
4. 13000rpm for 2 min,discard the flow-through
5. Put the collection tube into a clean EP tube, Add 50 uL 60C ddH2O in the center of the collection tube,standing for 1 min
6. 13000rpm for 1 min.
7. store the DNA solution at -20C