IGEM:Peking/2007/Count-Conjugation-Procedure

Make more independent riboregulators

• We choose to use <bbpart> pSB1A3 <\bbpart> and <bbpart> BBa_R0010 <\bbpart>(Plac) to express the taRNA, and <bbpart> pSB3K3 <\bbpart> and <bbpart> BBa_R0040 <\bbpart> to express <bbpart> BBa_E0040 <\bbpart>, whose translation is under the control of crRNA.
• crRNA is synthesized as two partially overlap ~50bp long primer, and one cycle PCR can make it to a dsDNA with some BioBrick cloning sites flanked.
• crRNA should be cut by NotI at 5' and SpeI at 3' and cloned into pSB1A3 to get the full length BioBrick prefix and suffix.
• First, we will try out the UC Berkley riboregulators <bbpart> BBa_J23078< \bbpart> and <bbpart> BBa_J23066 <\bbpart> first.
• This is the experimental procedure.