IGEM:Peking/2007/Count-Conjugation-Notebook/2007-7-23: Difference between revisions
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=== | ===Riboregulator=== | ||
Digested crRNA ( J23078 ) and R0040 for ligation tommorrow. | Digested crRNA ( J23078 ) and R0040 for ligation tommorrow. | ||
Revision as of 07:25, 23 July 2007
Riboregulator
Digested crRNA ( J23078 ) and R0040 for ligation tommorrow.
oriT Knock Out
- By Xu Anting
- Use chromosome extraction product as template to do PCR, with Taq or Pfu added.
- Result: In Taq PCR all 6 pairs of primers show bright bands at 500 bp, while none is shown in Pfu PCR.
- Conclusion: finally I got oriT up- and downstream fragments of 500 bp with potential site mutations caused by Taq's relatively low fidelity. I intend to use these products as template to do further PCR and get oriT-deleted fragments of 1200 bp.