IGEM:Peking/2007

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<div style="font: normal 1.2em georgia, serif">Towards Self-differentiated Bacterial Assembly Line</div><br>
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Our projects concern with the ability for bacterial cells to '''differentiate''' out of homogeneous conditions into populations with the '''division of labor'''. We aim at devices conferring host cells with the ability to form cooperating groups spontaneously and to take consecutive steps sequentially even when the genetic background and environmental inputs are identical. To break the mirror in such homogeneous condition, we need two devices respectively responsible for '''temporal''' and '''spatial''' differentiation. The implementation and application of such devices will lead to a picture of future bioengineering where complex programs consisted of sequential steps (structure oriented programs) and cooperating agencies (forked instances of a single class, object and event oriented) can be embedded in a single genome.
Our projects concern with the ability for bacterial cells to '''differentiate''' out of homogeneous conditions into populations with the '''division of labor'''. We aim at devices conferring host cells with the ability to form cooperating groups spontaneously and to take consecutive steps sequentially even when the genetic background and environmental inputs are identical. To break the mirror in such homogeneous condition, we need two devices respectively responsible for '''temporal''' and '''spatial''' differentiation. The implementation and application of such devices will lead to a picture of future bioengineering where complex programs consisted of sequential steps (structure oriented programs) and cooperating agencies (forked instances of a single class, object and event oriented) can be embedded in a single genome.
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About the name of projects, the two devices are named after their respective functions: hop count and push-on-push-off switch, but they are common for breaking symmetry, which is interesting and ubiquitous in science. Although this "differentiation" process resemble the development of multicellular organism, we tend to use a more bioengineering style analogy: assembly line. Or maybe after some years from now, this will not be just an analogy. <br>
About the name of projects, the two devices are named after their respective functions: hop count and push-on-push-off switch, but they are common for breaking symmetry, which is interesting and ubiquitous in science. Although this "differentiation" process resemble the development of multicellular organism, we tend to use a more bioengineering style analogy: assembly line. Or maybe after some years from now, this will not be just an analogy. <br>
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'''visit also:'''[http://parts.mit.edu/igem07/index.php/Peking | '''IGEM2007 Peking''']  
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'''Also visit:''' [http://parts.mit.edu/igem07/index.php/Peking '''IGEM2007 Peking''']  
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![http://parts.mit.edu/igem07/index.php/Peking_The_Community Peking The Team]
![http://parts.mit.edu/igem07/index.php/Peking_The_Community Peking The Team]
![http://parts.mit.edu/igem07/index.php/Peking_The_Fun Peking's Misc & Fun]
![http://parts.mit.edu/igem07/index.php/Peking_The_Fun Peking's Misc & Fun]
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![[iGEM:Peking/2007|Our OWW]]
 
![http://parts.mit.edu/igem07/index.php/Peking_Thanks Acknowledgement]
![http://parts.mit.edu/igem07/index.php/Peking_Thanks Acknowledgement]
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Some of our ideas are listed [[iGEM:Peking/2007/Ideas | here]]
Some of our ideas are listed [[iGEM:Peking/2007/Ideas | here]]
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'''Rap up'''<br>
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Collections of the experiences.
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* A [[IGEM:Peking/2007/Experience-colletcion | response]] to Goodman. In English.
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* Collections of [[IGEM:Peking/2007/Things_we_want_to_say |chinese sentiments]].
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*[[iGEM:Peking/2007/Chongyi Chen | Chongyi Chen]]
*[[iGEM:Peking/2007/Chongyi Chen | Chongyi Chen]]
*[[iGEM:Peking/2007/Chunbo Lou | Chunbo Lou]]
*[[iGEM:Peking/2007/Chunbo Lou | Chunbo Lou]]
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*[[iGEM:Peking/2007/Daizhuo Chen | Daizhuo Chen]]
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*[[User:Chen Daizhuo | Chen Daizhuo, Henry]]
*[[iGEM:Peking/2007/Dan Lu | Dan Lu]]
*[[iGEM:Peking/2007/Dan Lu | Dan Lu]]
*[[iGEM:Peking/2007/Haisu Ma | Haisu Ma]]
*[[iGEM:Peking/2007/Haisu Ma | Haisu Ma]]
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*[[iGEM:Peking/2007/Ting Liu | Ting Liu]]
*[[iGEM:Peking/2007/Ting Liu | Ting Liu]]
*[[iGEM:Peking/2007/Xili Liu | Xili Liu]]
*[[iGEM:Peking/2007/Xili Liu | Xili Liu]]
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*[[iGEM:Peking/2007/Yifan Yang | Yifan Yang]]
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*[[User:YangYifan | Yifan Yang]]
*[[iGEM:Peking/2007/Zhe Ren | Zhe Ren]]
*[[iGEM:Peking/2007/Zhe Ren | Zhe Ren]]

Current revision



Towards Self-differentiated Bacterial Assembly Line

Our projects concern with the ability for bacterial cells to differentiate out of homogeneous conditions into populations with the division of labor. We aim at devices conferring host cells with the ability to form cooperating groups spontaneously and to take consecutive steps sequentially even when the genetic background and environmental inputs are identical. To break the mirror in such homogeneous condition, we need two devices respectively responsible for temporal and spatial differentiation. The implementation and application of such devices will lead to a picture of future bioengineering where complex programs consisted of sequential steps (structure oriented programs) and cooperating agencies (forked instances of a single class, object and event oriented) can be embedded in a single genome.


About the name of projects, the two devices are named after their respective functions: hop count and push-on-push-off switch, but they are common for breaking symmetry, which is interesting and ubiquitous in science. Although this "differentiation" process resemble the development of multicellular organism, we tend to use a more bioengineering style analogy: assembly line. Or maybe after some years from now, this will not be just an analogy.
Also visit: IGEM2007 Peking


IGEM 2007 Peking Peking The Projects Peking The Team Peking's Misc & Fun Acknowledgement

Project Project

Hop Count with Conjugation
Project Proposal
Project Presentation
Road Map
Related Articles

Push-on-push-off Switch
Project proposal
Project presentation
Road Map
Related Articles

Meetings

Brainstorming
Some of our ideas are listed here

Rap up
Collections of the experiences.

News Lab

Products and Parts

Procedures
Hop-count with Conjugation
Push-on-push-off Switch

Protocols
Count:Agarose Gel Electrophoresis
Count:DNA Gel Extraction
Count:PCR product purifcation
Count:Preparation of Competent Cells
Enzymatic DNA Digestion
Count:Ligation
Count:Transformation
Count:Sequencing
Count: Miniprep
Count: Conjugation test
Count: Gene Knock Out
Switch: Miller Method
Oligonucleotides
Switch: PCR
Switch: DNA electrophoresis
Switch: DNA ligation
Switch: DNA digestion
Switch: transformation
Switch: DNA rescue by precipitation
Switch: Plasmid mini prep



Notebooks
Hop-count with Conjugation
Push-on-push-off Switch
Turtle Diary



People People

The iGEM 2007 PKU Team now consists of about 20 students and several faculty members from School of Life Science and Center of Theoretical Biology.

Students

Advisors

Photo

The Team

Our Campus

The Labs

Email us at igem-pku AT googlegroups DOT com

External links iGEM | iGEM 2006 | Registry of Standard Biological Parts | iGEM PKU Googlegroup | iGEM OWW Page

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