IGEM:Peking/2007: Difference between revisions
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'''Hop Count with Conjugation''' | '''Hop Count with Conjugation''' | ||
Hop counting is a cell-cell communication system in which the signals transfered among the cells record the number of times of transfer. We decided to use conjugation as our one to one cell-cell communication system and plasmids as the vector for the transfered signal. | Hop counting is a cell-cell communication system in which the signals transfered among the cells record the number of times of transfer. We decided to use conjugation as our one to one cell-cell communication system and plasmids as the vector for the transfered signal. | ||
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Besides intellectual interests, these systems are the core parts of controllable and encoded local cell-cell communication, which can be used in potential pattern formation and material weaving applications. <br> | Besides intellectual interests, these systems are the core parts of controllable and encoded local cell-cell communication, which can be used in potential pattern formation and material weaving applications. <br> | ||
'''Push-on-push-off Switch''' | '''Push-on-push-off Switch''' | ||
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</html>Project== | </html>Project== | ||
'''Hop Count with Conjugation''' | |||
[ftp://igem:igem2007@162.105.160.5/OurFiles/CountGroup/Documents/proposal.pdf Project Proposal]<br> | [ftp://igem:igem2007@162.105.160.5/OurFiles/CountGroup/Documents/proposal.pdf Project Proposal]<br> | ||
[ftp://igem:igem2007@162.105.160.5/OurFiles/CountGroup/Documents/presentation.pdf Project Presentation]<br> | [ftp://igem:igem2007@162.105.160.5/OurFiles/CountGroup/Documents/presentation.pdf Project Presentation]<br> | ||
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[[iGEM:Peking/2007/Count-References | Related Articles ]]<br> | [[iGEM:Peking/2007/Count-References | Related Articles ]]<br> | ||
'''Push_on_push_off Switch''' | |||
Project proposal<br> | Project proposal<br> | ||
Project presentation<br> | Project presentation<br> | ||
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<img src="http://openwetware.org/images/e/e2/Icon_info.png" alt="News" border="0"> | <img src="http://openwetware.org/images/e/e2/Icon_info.png" alt="News" border="0"> | ||
</html>Lab | </html>Lab== | ||
'''Products and Parts''' | '''Products and Parts''' | ||
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<img src="http://openwetware.org/images/3/39/Icon_groups.png" alt="People"> | <img src="http://openwetware.org/images/3/39/Icon_groups.png" alt="People"> | ||
</html>People | </html>People== | ||
The iGEM 2007 PKU Team now consists of about 20 students and several faculty members from School of Life Science and Center of Theoretical Biology. | The iGEM 2007 PKU Team now consists of about 20 students and several faculty members from School of Life Science and Center of Theoretical Biology. | ||
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[[iGEM:Peking/2007/Photo-Lab | The Labs]] | [[iGEM:Peking/2007/Photo-Lab | The Labs]] | ||
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'''Email us''' at ''igem-pku AT googlegroups DOT com'' | '''Email us''' at ''igem-pku AT googlegroups DOT com'' | ||
'''External links''' [http://parts.mit.edu/wiki/index.php/Main_Page iGEM] | [http://www.igem2006.com iGEM 2006] | [http://parts.mit.edu/ Registry of Standard Biological Parts] | [http://groups.google.com/group/igem-beijing/topics?lnk=oa iGEM PKU Googlegroup] | '''External links''' [http://parts.mit.edu/wiki/index.php/Main_Page iGEM] | [http://www.igem2006.com iGEM 2006] | [http://parts.mit.edu/ Registry of Standard Biological Parts] | [http://groups.google.com/group/igem-beijing/topics?lnk=oa iGEM PKU Googlegroup] | ||
Revision as of 20:01, 6 July 2007
Hop Count with Conjugation
Hop counting is a cell-cell communication system in which the signals transfered among the cells record the number of times of transfer. We decided to use conjugation as our one to one cell-cell communication system and plasmids as the vector for the transfered signal.
With the rolling-cycle replication of conjugation, independent tandem oriT (<bbpart>BBa_J01002</bbpart> and more) pairs can be inserted into the same plasmids, losing one tandem region at each single conjugation event.
Sequential conjugation and deletion of the different oriT pairs can be achieved by fine control of the expression of the cis-acting elements of the independent conjugation systems.
Besides intellectual interests, these systems are the core parts of controllable and encoded local cell-cell communication, which can be used in potential pattern formation and material weaving applications.
Push-on-push-off Switch
<html> <img src="http://openwetware.org/images/b/b9/Icon_board.png" alt="Project"> </html>ProjectHop Count with Conjugation
Project Proposal Push_on_push_off Switch
Project proposal Meetings
Brainstorming |
<html> <img src="http://openwetware.org/images/e/e2/Icon_info.png" alt="News" border="0"> </html>LabProducts and Parts Procedures Protocols Oligonucleotides Notebooks
|
<html> <img src="http://openwetware.org/images/3/39/Icon_groups.png" alt="People"> </html>PeopleThe iGEM 2007 PKU Team now consists of about 20 students and several faculty members from School of Life Science and Center of Theoretical Biology. StudentsPlease sign up here. Advisors
Photo |
Email us at igem-pku AT googlegroups DOT com
External links iGEM | iGEM 2006 | Registry of Standard Biological Parts | iGEM PKU Googlegroup
