Aleksandra
Transformation of TURBO cells with yesterday's ligations using 5µl ligation product to transform 50µl cells, then plating on Amp+LB with 150µl LB+transformation.
- mRBS/TetA(C)+RBS/LuxI
- wRBS/TetA(C)+RBS/LuxI
- stRBS/TetA(C)+RBS/LuxI
- stRBS/LacZα+RBS/LuxI
- RBS/mRFP/t(K081014)+pLux/GFP
- RBS/mRFP/tt(I13507)+pLux/GFP
- Pm/wRBS/LuxR +attC/term/attC/mRFP
- Ps/wRBS/LuxR +attC/term/attC/mRFP
- Pm/mRBS/luxR +attC/term/attC/mRFP
- Ps/mRBS/LuxR +attC/term/attC/mRFP
- Pm/sRBS/LuxR +attC/term/attC/mRFP
- Ps/sRBS/LuxR +attC/term/attC/mRFP
Controls:
- RBS/LuxI
- pLux/GFP
- Pm/wRBS/LuxR
- Ps/wRBS/LuxR
- Pm/mRBS/luxR
- Ps/mRBS/LuxR
- Pm/sRBS/LuxR
- Ps/sRBS/LuxR
- Positive control (pUC19)
All the transformation plates have colonies
verification of glycerols : plating
- wRBS/tetA(C)
- mRBS/TetA(C)
- stRBS/LacZα
- Pm
- Ps
- Ps/wRBS/LuxR
All plates have a lot of colonies, the glycerols are OK
Théotime
Make Plate 2x 500 mL of LBA + AMP : 2x 19 plates.
Aleksandra
Starting overnight cultures, 12ml cultures LB+Amp
- mRBS/TetA(C)+RBS/LuxI, 4 colonies
- wRBS/TetA(C)+RBS/LuxI, 4 colonies
- stRBS/TetA(C)+RBS/LuxI, 4 colonies
- RBS/mRFP/t(K081014)+pLux/GFP, 4 colonies
- RBS/mRFP/tt(I13507)+pLux/GFP, 4 colonies
- Pm/wRBS/LuxR +attC/term/attC/mRFP, 1 possible colony, didn't grow
- Ps/wRBS/LuxR +attC/term/attC/mRFP, 3 colonies
- Pm/mRBS/luxR +attC/term/attC/mRFP, 1 possible colony, didn't grow
- Ps/mRBS/LuxR +attC/term/attC/mRFP, 4 colonies
- Pm/sRBS/LuxR +attC/term/attC/mRFP, 1 colony
- Ps/sRBS/LuxR +attC/term/attC/mRFP, 4 colonies
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