IGEM:Paris Bettencourt 2012/Protocols/RCR Fusion

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Revision as of 03:51, 10 August 2012 by Denis. K. Samuylov (talk | contribs) (New page: * Pipetting instructions: {| border="1" class="wikitable" width="45%" | | <center>5x Phusion HF Buffer</center> | <center>10 ul</center> |- | <center>10 mM dNTPs</center> | <center>1 uL</...)
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  • Pipetting instructions:
5x Phusion HF Buffer
10 ul
10 mM dNTPs
1 uL
Forward primer
0.1 - 1.0 uM
Reverse primer
0.1 - 1.0 uM
Template DNA
10 pg - 1 ug
Water (Nuclease free)
to 50 ul
Total volume:
50 ul
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