IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/07/11

(Difference between revisions)

Revision as of 05:07, 12 July 2012

Project name

Ligation (after digested all with X and P):
- pSG.001 as negative control
- pSG.002 as negative control
- pSG.005 (pSG.001+pRG.006 (pLac from Antoine))
- psG.006 (pSG.002+pSG.004 (pTet from kit))
Transforming the ligation product and plating (plus 1 positive control)
Digesting pSG.004 and pRG.006 with X and P

@@ Line 8: / Line 8: @@
 ==What we did today==
 *Ligation (after digested all with X and P):
-**pSG001 as negative control
+**pSG.001 as negative control
-**pSG002 as negative control
+**pSG.002 as negative control
-**pSG005 (pSG001+pRG006 (pLac from Antoine))
+**pSG.005 (pSG.001+pRG.006 (pLac from Antoine))
-**psG006 (pSG002+pSG004 (pTet from kit))
+**psG.006 (pSG.002+pSG.004 (pTet from kit))
-*Transforming the ligation product and plating
+*Transforming the ligation product and plating (plus 1 positive control)
-*Digesting pSG004 and pRG006 with X and P
+*Digesting pSG.004 and pRG.006 with X and P
 [[Image:110712.jpg]]
-*Growing more pSG004 and pRG006
+*Growing more pSG.004 and pRG.006