IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/07/11

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**pSG001 as negative control
**pSG001 as negative control
**pSG002 as negative control
**pSG002 as negative control
-
**pSG005 (psG001+pRG006 (pLac from Antoine))
+
**pSG005 (pSG001+pRG006 (pLac from Antoine))
**psG006 (pSG002+pSG004 (pTet from kit))
**psG006 (pSG002+pSG004 (pTet from kit))
*Transforming the ligation product and plating
*Transforming the ligation product and plating

Revision as of 05:03, 12 July 2012

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What we did today

  • Ligation (after digested all with X and P):
    • pSG001 as negative control
    • pSG002 as negative control
    • pSG005 (pSG001+pRG006 (pLac from Antoine))
    • psG006 (pSG002+pSG004 (pTet from kit))
  • Transforming the ligation product and plating
  • Digesting pSG004 and pRG006 with X and P


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