IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/07/11
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==What we did today== | ==What we did today== | ||
| - | * | + | *Ligated (after digested all with X and P): |
**pSG.001 as negative control | **pSG.001 as negative control | ||
**pSG.002 as negative control | **pSG.002 as negative control | ||
**pSG.005 (pSG.001+pRG.006 (pLac from Antoine)) | **pSG.005 (pSG.001+pRG.006 (pLac from Antoine)) | ||
**psG.006 (pSG.002+pSG.004 (pTet from kit)) | **psG.006 (pSG.002+pSG.004 (pTet from kit)) | ||
| - | * | + | *Transformed the ligation product and plating (plus 1 positive control) |
| - | * | + | *Digested pSG.004 and pRG.006 with X and P then extracted the gel |
[[Image:110712.jpg]] | [[Image:110712.jpg]] | ||
| - | * | + | *Grew more pSG.004 and pRG.006 |
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