IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/07/21
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Making -20°C & -80°C stocks
Miniprep of : pSC001a, pSC002a&b, pSC003a
->the big amount of lysis solution did not perturb the miniprep.
Digestion of pSC001(a) & pSC002(a)
--> 30 min, 37°C water bath.
Migration on 1% agarose gel
Migration of 10µL of the digestions
The bands are as expected, we can PCR purif the digestion (2 tubes of 30 µL)
The PCR purif is made with the promega kit, I recover dna in 30 µL of water.
Ligation of pSC002 dig. + pSC003 dig.