IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/07/21

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Making -20°C & -80°C stocks

  • I saved 750µL of O/N culture, in order to add 250µL of 60% glycerol sterile, in sterile tubes. I don't find them. I put the 750µL in normal eppendorf, at 4°C.

Miniprep of : pSC001a, pSC002a&b, pSC003a

  • I used 500µL of Lysis solution instead of 250µL
  • Recover plasmid in 70µL of water.
  • Nanodrop :
    • pSC001a : 186,4 ng/µL
    • pSC002a : 157,1 ng/µL
    • pSC002b : 144 ng/µL
    • pSC003a : 142,4 ng/µL

->the big amount of lysis solution did not perturb the miniprep.



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