IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/10/16: Difference between revisions
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==PCR QCM - 2nd part== | ==PCR QCM - 2nd part== | ||
* I digested the PCR product with DpnI for 1h, then I transformed either 1, 3 or 5µL of digested product in NEB and MG1655 cells. | |||
* I add 200µL of LB and then let recovering at 37°C for 1h. | |||
I plated everything on LB+Amp, and let it grow O/N at 37°C. | |||
==Soil sample test== | |||
Revision as of 08:37, 16 October 2012
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PCR QCM - 2nd part
I plated everything on LB+Amp, and let it grow O/N at 37°C. Soil sample test | |
