IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/07/10/Zhangs

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Electrophoresis 12 samples of PCR identification products.
Sample loading order: T7p+5N+CI+T①~⑤, T7P+2G+CI+T①~⑤, T7P+2I+CI+TL①~②, Marker

The T7P+5N+CI+T①-⑤ & T7P+2G+CI+T①-⑤ & T7P+2I+CI+T①,are target spawns (PCR identified).

Electrophoresis another 12 samples of PCR identification products.
Sample loading order: T7p+2I+CI+T③~⑤, T7P+2M+CI+T①~⑤, T7P+11N+CI+T①~④, Marker

The T7P+2I+CI+T③-⑤ & T7P+2M+CI+T①-⑤ & T7P+11N+CI+T②-④ are target spawns (PCR identified).
Electrophoresis another 12 samples of PCR identification products.
Sample loading order: T7p+11N+CI+T⑤, T7P+1J+CI+T①~⑤, T7P+1H+CI+T①~⑤, LinMIin, Marker

The T7P+1J+CI+T①-⑤ & T7P+1H+CI+T④ are target spawns (PCR identified).

Transfer the colonies into tubes with 5mL LB:
T7P+1J+CI+T①;
T7P+5N+CI+T①;
T7P+2G+CI+T①;
T7P+2I+CI+T①;
T7P+2M+CI+T①;
T7P+11N+CI+T②;
T7P+1H+CI+T④.

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