IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/10/03: Difference between revisions

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(Autocreate 2014/10/03 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue)
 
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b> ==
==<b>Date: 10/3/14</b> <b>People in lab: Kelsey Crossen</b>==
 
<b><span style="font-size:16px;">Title:</span></b> Broth cultures of CT1-6 10/2
<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.
<b>Start Time:</b> 2 pm
 
<b>Purpose:</b> Miniprep preparation
<b>Start Time:</b>
<b>Protocol:</b> LTM Ed. 2 Innoculation
 
<b>Exceptions:</b>  
<b>Purpose:</b>  
 
<b>Protocol:</b> <b>Exceptions:</b>  
 
<b>Products:</b>  
<b>Products:</b>  
{| border="1" cellpadding="5"
{| border= " 1 " cellpadding = " 5 "  
|-
! Sample Label !! Description !! Source Label !! Quantity
|-
| BC1 10/3 || hmp psB1C3 || CT2 10/2 || 1
|-
| BC2 10/3 || hmp psB1C3 || CT1 10/2 || 1
|-
| BC3 10/3 || hmp psB1C3 || CT1 10/2 || 1
|-
| BC4 10/3 || hmp with pro+RBS || CT1 10/3 || 1
|-
| BC5 10/3 || hmp with pro+RBS || CT3 10/2 || 1
|-
| BC6 10/3 || hmp with pro+RBS || CT3 10/2 || 1
|-
| BC7 10/3 || hmp with pro+RBS || CT4 10/2 || 1
|-
|-
! Sample Label !! Description !! Source Label !! Quantity
| BC8 10/3 || hmp with pro+RBS || CT4 10/2 || 1
|-
|-
| Entry || Entry || Entry || #
| BC9 10/3 || hmp with pro+RBS || CT5 10/2 || 1
|-
|-
| Entry || Entry || Entry || #
| BC10 10/3 || hmp with pro+RBS || CT5 10/2 || 1
|}
 
{| border="1" cellpadding="5"
|-
|-
! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
| BC11 10/3 || hmp with pro+RBS || CT6 10/2 || 1
|-
|-
| Sample || || ||  ||  ||  ||  ||  ||  
| BC12 10/3 || hmp with pro+RBS || CT6 10/2 || 1
|}  
|}
 
<b>Results:</b>  
<b>Results:</b>  
<b>Notes:</b>  
<b>Notes:</b>  
<b>Stop Time:</b> 2:45 pm
<b>Next:</b> Glycerol stocks of BC1-3 and miniprep of the rest


<b>Stop Time:</b>  
==<b>Date: 10/3/14</b> <b>People in lab: Kira Buckowing</b>==
<b><span style="font-size:16px;">Title:</span></b> Chemical Transformation of L1 and L2 10/2
<b>Start Time:</b> 8:30 pm
<b>Purpose:</b> To test if the digestion and ligation of norV was successful
<b>Protocol:</b> LTM Ed. 2 Chemical Transformation
<b>Exceptions:</b>
<b>Products:</b>
{| border= " 1 " cellpadding = " 5 "
|-
! Sample Label !! Description !! Source Label !! Quantity
|-
| CT1 10/3 || 20 uL || L1 10/2 || 1
|-
| CT2 10/3 || 200 uL || L1 10/2 || 1
|-
| CT3 10/3 || 20 uL || L2 10/2 || 1
|-
| CT4 10/3 || 200 uL || L2 10/2 || 1


<b>Next:</b>


|}
<b>Results:</b>
<b>Notes:</b> Wrong plates used by mistake at the last step and the procedure was started over and redone (original start time ~5 pm)
<b>Stop Time:</b> 11:30 pm
<b>Next:</b> Innoculation of any colonies that grow




Revision as of 20:17, 14 October 2014

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Date: 10/3/14 People in lab: Kelsey Crossen

Title: Broth cultures of CT1-6 10/2 Start Time: 2 pm Purpose: Miniprep preparation Protocol: LTM Ed. 2 Innoculation Exceptions: Products:

Sample Label Description Source Label Quantity
BC1 10/3 hmp psB1C3 CT2 10/2 1
BC2 10/3 hmp psB1C3 CT1 10/2 1
BC3 10/3 hmp psB1C3 CT1 10/2 1
BC4 10/3 hmp with pro+RBS CT1 10/3 1
BC5 10/3 hmp with pro+RBS CT3 10/2 1
BC6 10/3 hmp with pro+RBS CT3 10/2 1
BC7 10/3 hmp with pro+RBS CT4 10/2 1
BC8 10/3 hmp with pro+RBS CT4 10/2 1
BC9 10/3 hmp with pro+RBS CT5 10/2 1
BC10 10/3 hmp with pro+RBS CT5 10/2 1
BC11 10/3 hmp with pro+RBS CT6 10/2 1
BC12 10/3 hmp with pro+RBS CT6 10/2 1

Results: Notes: Stop Time: 2:45 pm Next: Glycerol stocks of BC1-3 and miniprep of the rest

Date: 10/3/14 People in lab: Kira Buckowing

Title: Chemical Transformation of L1 and L2 10/2 Start Time: 8:30 pm Purpose: To test if the digestion and ligation of norV was successful Protocol: LTM Ed. 2 Chemical Transformation Exceptions: Products:

Sample Label Description Source Label Quantity
CT1 10/3 20 uL L1 10/2 1
CT2 10/3 200 uL L1 10/2 1
CT3 10/3 20 uL L2 10/2 1
CT4 10/3 200 uL L2 10/2 1


Results: Notes: Wrong plates used by mistake at the last step and the procedure was started over and redone (original start time ~5 pm) Stop Time: 11:30 pm Next: Innoculation of any colonies that grow



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