IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/08/09: Difference between revisions

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(Autocreate 2014/08/09 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue)
 
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b> ==
==<b>Date: 8/9/14</b> <b>People in lab: Emily Puleo, Joshua Heath</b>==
 
<b><span style="font-size:16px;">Title:</span></b> Miniprep and Nanodrop
<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.
<b>Start Time:</b> 10:15 am
 
<b>Purpose:</b> Purify plasmid DNA and measure purity
<b>Start Time:</b>
<b>Protocol:</b> LTM Ed. 2 Miniprep and Nanodrop
 
<b>Exceptions:</b>  
<b>Purpose:</b>  
 
<b>Protocol:</b> <b>Exceptions:</b>  
 
<b>Products:</b>  
<b>Products:</b>  
{| border="1" cellpadding="5"
{| border= " 1 " cellpadding = " 5 "  
|-
|-
! Sample Label !! Description !! Source Label !! Quantity
! Sample Label !! Description !! Source Label !! Quantity  
|-
|-
| Entry || Entry || Entry || #
| 8/9 MP1 || ng/uL = 52.1; 260/280 = 2.1; 260/230 = 2.05 || 8/8 BC1 || 1
|-
|-
| Entry || Entry || Entry || #
| 8/9 MP2 || ng/uL = 58.7; 260/280 = 2.11; 260/230 = 2.07 || 8/8 BC2 || 1
|}
 
{| border="1" cellpadding="5"
|-
|-
! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
| 8/9 MP3 || ng/uL = 58.1; 260/280 = 2.1; 260/230 = 2.05 || 8/8 BC3 || 1
|-
|-
| Sample || || || ||  ||  ||  ||  ||
| 8/9 MP4 || ng/uL = 51.4; 260/280 = 2.05; 260/230 = 1.98 || 8/8 BC4 || 1
|}


|}
<b>Results:</b>  
<b>Results:</b>  
 
<b>Notes:</b> When 8/8 BC4 was being taken out of the incubator, the lid came off
<b>Notes:</b>  
<b>Stop Time:</b> 12:30 pm
 
<b>Next:</b>  
<b>Stop Time:</b>  
 
<b>Next:</b>




Revision as of 14:24, 14 October 2014

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Date: 8/9/14 People in lab: Emily Puleo, Joshua Heath

Title: Miniprep and Nanodrop Start Time: 10:15 am Purpose: Purify plasmid DNA and measure purity Protocol: LTM Ed. 2 Miniprep and Nanodrop Exceptions: Products:

Sample Label Description Source Label Quantity
8/9 MP1 ng/uL = 52.1; 260/280 = 2.1; 260/230 = 2.05 8/8 BC1 1
8/9 MP2 ng/uL = 58.7; 260/280 = 2.11; 260/230 = 2.07 8/8 BC2 1
8/9 MP3 ng/uL = 58.1; 260/280 = 2.1; 260/230 = 2.05 8/8 BC3 1
8/9 MP4 ng/uL = 51.4; 260/280 = 2.05; 260/230 = 1.98 8/8 BC4 1


Results: Notes: When 8/8 BC4 was being taken out of the incubator, the lid came off Stop Time: 12:30 pm Next:



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