IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/07/30

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Date: 7/30/14 People in lab: Joshua Heath

Title: Miniprep and Nanodrop Start Time: 10:40 am Purpose: Purify DNA and check quality Protocol: LTM Ed. 2 Miniprep and Nanodrop Exceptions: Products:

Sample Label Description Source Label Quantity
7/30 MP 1 Water 7/29 BC1 1
7/30 MP 2 Water 7/29 BC2 1
7/30 MP 3 Elution 7/29 BC3 1
7/30 MP 4 Elution 7/29 BC4 1


Results: MP 1 - ng/uL = 171.1, 260/280 = 1.92, 260/230 = 3.02; MP 2 ng/uL = 171.6, 260/280 = 1.9, 260/230 = 3.17; MP 3 ng/uL = 161.2, 260/280 = 1.92, 260/230 = 2.21; MP 4 ng/uL = 186.1, 260/280 = 1.9, 260/230 = 2.15 Notes: Stop Time: 1:40 pm Next: Digest with EcoRI