IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/07/30: Difference between revisions

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(Autocreate 2014/07/30 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue)
 
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b> ==
==<b>Date: 7/30/14</b> <b>People in lab: Joshua Heath</b>==
 
<b><span style="font-size:16px;">Title:</span></b> Miniprep and Nanodrop
<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.
<b>Start Time:</b> 10:40 am
 
<b>Purpose:</b> Purify DNA and check quality
<b>Start Time:</b>
<b>Protocol:</b> LTM Ed. 2 Miniprep and Nanodrop
 
<b>Exceptions:</b>  
<b>Purpose:</b>  
 
<b>Protocol:</b> <b>Exceptions:</b>  
 
<b>Products:</b>  
<b>Products:</b>  
{| border="1" cellpadding="5"
{| border= " 1 " cellpadding = " 5 "  
|-
|-
! Sample Label !! Description !! Source Label !! Quantity
! Sample Label !! Description !! Source Label !! Quantity  
|-
|-
| Entry || Entry || Entry || #
| 7/30 MP 1 || Water || 7/29 BC1 || 1
|-
|-
| Entry || Entry || Entry || #
| 7/30 MP 2 || Water || 7/29 BC2 || 1
|}
 
{| border="1" cellpadding="5"
|-
|-
! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
| 7/30 MP 3 || Elution || 7/29 BC3 || 1
|-
|-
| Sample || || || ||  ||  ||  ||  ||
| 7/30 MP 4 || Elution || 7/29 BC4 || 1
|}


<b>Results:</b>


|}
<b>Results:</b> MP 1 - ng/uL = 171.1, 260/280 = 1.92, 260/230 = 3.02; MP 2 ng/uL = 171.6, 260/280 = 1.9, 260/230 = 3.17; MP 3 ng/uL = 161.2, 260/280 = 1.92, 260/230 = 2.21; MP 4 ng/uL = 186.1, 260/280 = 1.9, 260/230 = 2.15
<b>Notes:</b>  
<b>Notes:</b>  
 
<b>Stop Time:</b> 1:40 pm
<b>Stop Time:</b>  
<b>Next:</b> Digest with EcoRI
 
<b>Next:</b>
 





Revision as of 21:59, 13 October 2014

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Date: 7/30/14 People in lab: Joshua Heath

Title: Miniprep and Nanodrop Start Time: 10:40 am Purpose: Purify DNA and check quality Protocol: LTM Ed. 2 Miniprep and Nanodrop Exceptions: Products:

Sample Label Description Source Label Quantity
7/30 MP 1 Water 7/29 BC1 1
7/30 MP 2 Water 7/29 BC2 1
7/30 MP 3 Elution 7/29 BC3 1
7/30 MP 4 Elution 7/29 BC4 1


Results: MP 1 - ng/uL = 171.1, 260/280 = 1.92, 260/230 = 3.02; MP 2 ng/uL = 171.6, 260/280 = 1.9, 260/230 = 3.17; MP 3 ng/uL = 161.2, 260/280 = 1.92, 260/230 = 2.21; MP 4 ng/uL = 186.1, 260/280 = 1.9, 260/230 = 2.15 Notes: Stop Time: 1:40 pm Next: Digest with EcoRI