IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/07/30: Difference between revisions
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(Autocreate 2014/07/30 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue) |
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== <b>Date: | ==<b>Date: 7/30/14</b> <b>People in lab: Joshua Heath</b>== | ||
<b><span style="font-size:16px;">Title:</span></b> Miniprep and Nanodrop | |||
<b><span style="font-size:16px;">Title:</span></b> | <b>Start Time:</b> 10:40 am | ||
<b>Purpose:</b> Purify DNA and check quality | |||
<b>Start Time:</b> | <b>Protocol:</b> LTM Ed. 2 Miniprep and Nanodrop | ||
<b>Exceptions:</b> | |||
<b>Purpose:</b> | |||
<b>Protocol:</b> <b>Exceptions:</b> | |||
<b>Products:</b> | <b>Products:</b> | ||
{| border="1" cellpadding="5" | {| border= " 1 " cellpadding = " 5 " | ||
|- | |- | ||
! Sample Label !! Description !! Source Label !! Quantity | ! Sample Label !! Description !! Source Label !! Quantity | ||
|- | |- | ||
| | | 7/30 MP 1 || Water || 7/29 BC1 || 1 | ||
|- | |- | ||
| | | 7/30 MP 2 || Water || 7/29 BC2 || 1 | ||
|- | |- | ||
| 7/30 MP 3 || Elution || 7/29 BC3 || 1 | |||
|- | |- | ||
| | | 7/30 MP 4 || Elution || 7/29 BC4 || 1 | ||
|} | |||
<b>Results:</b> MP 1 - ng/uL = 171.1, 260/280 = 1.92, 260/230 = 3.02; MP 2 ng/uL = 171.6, 260/280 = 1.9, 260/230 = 3.17; MP 3 ng/uL = 161.2, 260/280 = 1.92, 260/230 = 2.21; MP 4 ng/uL = 186.1, 260/280 = 1.9, 260/230 = 2.15 | |||
<b>Notes:</b> | <b>Notes:</b> | ||
<b>Stop Time:</b> 1:40 pm | |||
<b>Stop Time:</b> | <b>Next:</b> Digest with EcoRI | ||
<b>Next:</b> | |||
Revision as of 21:59, 13 October 2014
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Date: 7/30/14 People in lab: Joshua HeathTitle: Miniprep and Nanodrop Start Time: 10:40 am Purpose: Purify DNA and check quality Protocol: LTM Ed. 2 Miniprep and Nanodrop Exceptions: Products:
Results: MP 1 - ng/uL = 171.1, 260/280 = 1.92, 260/230 = 3.02; MP 2 ng/uL = 171.6, 260/280 = 1.9, 260/230 = 3.17; MP 3 ng/uL = 161.2, 260/280 = 1.92, 260/230 = 2.21; MP 4 ng/uL = 186.1, 260/280 = 1.9, 260/230 = 2.15 Notes: Stop Time: 1:40 pm Next: Digest with EcoRI
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