IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/07/24: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 31: Line 31:
<b>Stop Time:</b> 3:10 pm
<b>Stop Time:</b> 3:10 pm
<b>Next:</b> TOPO
<b>Next:</b> TOPO
==<b>Date: 7/24/14</b> <b>People in lab: Emily Puleo, Caleb Treccazi</b>==
<b><span style="font-size:16px;">Title:</span></b> TOPO cloning of hmp and transformations of hmp and norV
<b>Start Time:</b> 3:10 pm
<b>Purpose:</b> To get lots of copies of hmp and do blue white screening and to test ligated norV product
<b>Protocol:</b> LTM Ed 2 Transformations, Invitrogen TOPO cloning kit manual
<b>Exceptions:</b>
<b>Products:</b>
{| border= " 1 " cellpadding = " 5 "
|-
! Sample Label !! Description !! Source Label !! Quantity
|-
| 7/24 CT hmp 1 || 20 uL hmp and 60 uL xgal || 7/24 A || 1
|-
| 7/24 CT hmp 2 || 200 uL hmp and 60 uL xgal || 7/24 A || 1
|-
| 7/24 CT norV 1 || 20 uL || L1 6/6 || 1
|-
| 7/24 CT norV 2 || 200 uL || L1 6/6 || 1
|}
<b>Results:</b> (7/25) No growth except on hmp 1 where there were lots of white colonies and one blue colony
<b>Notes:</b>
<b>Stop Time:</b> 5:45 pm
<b>Next:</b> blue white screening of hmp; innoculation of norV from any growth


<!-- ## Do not edit below this line unless you know what you are doing. ## -->
<!-- ## Do not edit below this line unless you know what you are doing. ## -->

Revision as of 21:55, 13 October 2014

Cyanobacteria Mediated Filtration of Coal Flue Gas <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Date: 7/24/14 People in lab: Emily Puleo

Title: PCR hmp Start Time: 12:03 pm Purpose: Add prefix and suffix Protocol: LTM Ed 2 PCR Exceptions: 2.5 uL primers, 7.5 uL E coli Template Products:

Sample Label Description Source Label Quantity
7/24 A hmp with prefix and suffix added --- 1





Results: Notes: Stop Time: 3:10 pm Next: TOPO

Date: 7/24/14 People in lab: Emily Puleo, Caleb Treccazi

Title: TOPO cloning of hmp and transformations of hmp and norV Start Time: 3:10 pm Purpose: To get lots of copies of hmp and do blue white screening and to test ligated norV product Protocol: LTM Ed 2 Transformations, Invitrogen TOPO cloning kit manual Exceptions: Products:

Sample Label Description Source Label Quantity
7/24 CT hmp 1 20 uL hmp and 60 uL xgal 7/24 A 1
7/24 CT hmp 2 200 uL hmp and 60 uL xgal 7/24 A 1
7/24 CT norV 1 20 uL L1 6/6 1
7/24 CT norV 2 200 uL L1 6/6 1


Results: (7/25) No growth except on hmp 1 where there were lots of white colonies and one blue colony Notes: Stop Time: 5:45 pm Next: blue white screening of hmp; innoculation of norV from any growth



Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue/2014/07/24&oldid=828571"